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与MiSeq使用MiSeq Reporter自动将二进制碱基调用（BCL）文件转换为FASTQ格式不同，NextSeq、HiSeq和NovaSeq系统的输出需要用户开发的或第三方的数据分析工具，如bcl2fastq，来转换为FASTQ。除了…

LoadH5Seurat报错 Error in if (!x[[i]]$dims) { : argument is of length zero 解决： 添加 assays = “RNA” 或 array = &#8…

报错信息 > install.packages(“rgeos”) Installing package into ‘/home/jody/R/x86_64-pc-linux-gnu-library/3.4’ (as ‘lib’ is …

1.配置Conda镜像源 conda config –add channels https://mirrors.tuna.tsinghua.edu.cn/anaconda/pkgs/free conda config –add chan…

修改命令：sudo gedit /etc/apt/sources.list 阿里源 deb http://mirrors.aliyun.com/ubuntu/ bionic main restricted universe multiver…

1.安装curl/wget curl -o cellranger-6.1.2.tar.gz “https://cf.10xgenomics.com/releases/cell-exp/cellranger-6.1.2.tar.gz?Expi…

安装包 install.packages(“remotes”)remotes::install_github(“mojaveazure/seurat-disk”,force = TRUE) 加…

require(DOSE) require(clusterProfiler) data(geneList) gene = names(geneList)[abs(geneList) > 2] david = enrichDAVID(g…