bio
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PBST缓冲液配制方法
1X PBST Solution concentrations: NaCl: 137 mM KCl: 2.7 mM Na2HPO4: 10 mM KH2PO4: 1.8 mM Tween® 20 detergent: 0.1% (w/v) …
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Western Blot：TBST缓冲液配制方法
含 0.1% Tween 20 TBST是许多免疫测定的有效洗涤缓冲液。要配制 1 升 TBST 冲洗缓冲液，可在 900 毫升水中加入 100 毫升 10X TBS 和 1 毫升 Tween 20 。 1X Solution concen…
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Western Blot：Tris-Buffered Saline (TBS) 10X Stock Solution配制方法
TBS是用于多种类型免疫测定的极佳洗涤缓冲液。要配制 1 升 10 倍 TBS 缓冲溶液，可将 24 克 Tris 和 88 克 NaCl 溶于 900 毫升水中，然后将 pH 值调至 7.6，最终体积为 1 升。 Solution con…
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Western Blot：4X SDS Sample Loading Buffer上样缓冲液配方
SDS 样品上样缓冲液配方是制备蛋白质样品并将其上样到凝胶中进行聚丙烯酰胺凝胶电泳分析的理想选择。 4X Solution concentrations: Tris-HCl: 0.2 M DTT: 0.4 M SDS: 277 mM, 8.…
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药物开发的通用公共数据库
数据库名称 描述 URL dbSNP SNPs for a wide range of organisms, including >150M human reference SNPs. http://www.ncbi.nlm.nih.…
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分析错误: Calcium-dependent transcriptional changes in human pancreatic islet cells reveal functional diversity in islet cell subtypes
Diabetologia；撤稿日期：04 August 2023 The authors have retracted this article following the discovery of a data proces…
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重复用图：microRNA-133a exerts tumor suppressive role in oral squamous cell carcinoma through the Notch signaling pathway via downregulation of CTBP2
microRNA-133a exerts tumor suppressive role in oral squamous cell carcinoma through the Notch signaling pathway via down…
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重复用图:MiRNA-211 suppresses cell proliferation, migration and invasion by targeting SPARC in human hepatocellular carcinoma
MiRNA-211 suppresses cell proliferation, migration and invasion by targeting SPARC in human hepatocellular carcinoma Sci…
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重复用图: MicroRNA-9 modified bone marrow-derived mesenchymal stem cells (BMSCs) repair severe acute pancreatitis (SAP) via inducing angiogenesis in rats
MicroRNA-9 modified bone marrow-derived mesenchymal stem cells (BMSCs) repair severe acute pancreatitis (SAP) via induci…
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Frontiers系列期刊2017-2021拒稿率，2021达40%
Frontiers不设定接受率或拒稿率。通过严格同行评审的文章会被录用，未通过评审的文章会被拒稿。 随着投稿量逐年增加，拒稿率也在不断上升。2021 年，所有学科 40% 的投稿都被拒稿，部份期刊的拒稿率超过 60%。 虽然 APC 只适用…
