单细胞测序
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10X Genomics 单细胞 RNA 测序（scRNA-seq）文库制备协议: 10XV2 和 10XV3
10X Genomics 的 10XV2 和 10XV3 是两种单细胞 RNA 测序（scRNA-seq）文库制备协议，主要区别如下： 1. 化学试剂版本 10XV2: 使用的是第二代化学试剂（v2 chemistry）。 10XV3: 使…
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single-cell data analysis software packages
awesome-single-cell List of software packages (and the people developing these methods) for single-cell data analysis, i…
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R:integrating and analysis single-cell datasets
Introduction In this notebook I will go over several integration techniques for single-cell -omics data. These technique…
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Cellranger分析10X Genomics single-cell RNA-seq data单细胞原始数据
cellranger是处理从 10X Genomics 平台生成的单细胞 RNA-seq 数据的命令行工具。cellranger 由 10X Genomics 构建，专门用于处理从其平台生成的数据，然而有几种开源工具可以与 cellrang…
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R: Load in data from 10X
Description Enables easy loading of sparse data matrices provided by 10X genomics. Usage Read10X( data.dir, gene.column …
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VISION：Functional Interpretation for scRNA-seq Data
Preliminaries If you have yet to install VISION, we recommend installing the package from Github to install this package…
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Developmental cell:整合单细胞蛋白质组学和转录组学的人类乳腺图谱-A human breast atlas integrating single-cell proteomics and transcriptomics
Published:May 25, 2022DOI:https://doi.org/10.1016/j.devcel.2022.05.003 The breast is a dynamic organ whose response to p…
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在Ubuntu 18.04上编译bcl2fastq v2.20
与MiSeq使用MiSeq Reporter自动将二进制碱基调用（BCL）文件转换为FASTQ格式不同，NextSeq、HiSeq和NovaSeq系统的输出需要用户开发的或第三方的数据分析工具，如bcl2fastq，来转换为FASTQ。除了…
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conda 安装R语言及其R
1.配置Conda镜像源 conda config –add channels https://mirrors.tuna.tsinghua.edu.cn/anaconda/pkgs/free conda config –add chan…
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Cell Ranger – 6.1.2安装和使用
1.安装curl/wget curl -o cellranger-6.1.2.tar.gz “https://cf.10xgenomics.com/releases/cell-exp/cellranger-6.1.2.tar.gz?Expi…
