SDS 样品上样缓冲液配方是制备蛋白质样品并将其上样到凝胶中进行聚丙烯酰胺凝胶电泳分析的理想选择。
4X Solution concentrations:
- Tris-HCl: 0.2 M
- DTT: 0.4 M
- SDS: 277 mM, 8.0% (w/v)
- Bromophenol blue: 6 mM
- Glycerol: 4.3 M
100mL储存液配制方法:
20 mL 1 M Tris-HCl pH 6.5 |
3 mL of 1 M DTT (dithiothreitol) |
0.6 g of SDS (sodium dodecyl sulfate) |
30 mg of bromophenol blue |
2.4 mL of glycerol |
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