| AAV碘克沙醇纯化法常用试剂配制浓度 | ||
| 5 M NaCl | 146.1 g NaCl | |
| Up to 500 mL Ultrapure water | ||
| 1 M Tris HCl (pH 8.5) | 12.11 g Tris base | |
| Up to 100 mL Ultrapure water | ||
| Add 1 M HCl using a Pasteur pipette to reduce the pH to 8.5 | ||
| Lysis buffer | 15 mL of 5 M NaCl | |
| 25 mL of 1 M Tris HCl (pH 8.5) | ||
| Up to 500 mL Ultrapure water | ||
| 10x Phosphate-buffered saline (PBS) | 80 g NaCl | |
| 2 g KCl | ||
| 14.4 g Na2HPO4 | ||
| 2.4 g KH2PO4 | ||
| Up to 1 L ddwater | ||
| 1 M MgCl2 | 20.33 g MgCl2-6H2O | |
| Up to 100 mL Ultrapure water | ||
| 1 M KCl | 7.45 g KCl | |
| Up to 100 mL Ultrapure water | ||
| PBS-MK: 5x PBS Magnesium-Potassium stock solution | 250 mL of 10x PBS | |
| 2.5 mL of 1 M MgCl2 | ||
| 6.25 mL of 1 M KCl | ||
| Up to 500 mL Ultrapure water H2O | ||
| 15% Iodixanol | 12.5 mL of Optiprep density gradient medium(Sigma-Aldrich,D1556) | |
| 10 mL of 5 M NaCl | ||
| 10 mL of 5x PBS-MK | ||
| 17.5 mL of Ultrapure water | ||
| 25% Iodixanol | 20.8 mL of Optiprep density gradient medium | |
| 10 mL of 5x PBS-MK | ||
| 19.2 mL of Ultrapure water | ||
| 100 µL of phenol red | ||
| 40% Iodixanol | 33.3 mL of Optiprep density gradient medium | |
| 10 mL of 5x PBS-MK | ||
| 6.7 mL of Ultrapure water | ||
| 60% Iodixanol | 50 mL of Optiprep density gradient medium | |
| 100 µL of phenol red | ||
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