试剂 | 终浓度 | 500 mL需要量 |
---|---|---|
RPMI 1640 containing 11.11 mM glucose | 439 mL | |
Fetal Bovine Serum | 10% | 50 mL |
Glutamax, 200 mM | 2 mM | 5 mL |
Streptomycin/Penicillin 10′000 U/mL / 10′000 μg/mL | 100 U/mL / 100μg/mL | 5 mL |
Amphotericin B, 250 μg/mL | 0.25 μg/mL | 0.5 mL |
Gentamycin, 50 mg/mL | 50 μg/mL | 0.5 mL |
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