错误:
STAR –runThreadN 12 –twopassMode Basic –genomeDir /home/users/admin/common_files/hg38/STAR –readFilesIn /home/users/admin/1728-01-151_1.fq /home/users/admin/1728-01-151_2.fq –outSAMtype BAM SortedByCoordinate
And error is related to Genome version.
EXITING because of FATAL ERROR: Genome version: 20201 is INCOMPATIBLE with running STAR version: 2.7.5a_2020-06-29
SOLUTION: please re-generate genome from scratch with running version of STAR, or with version: 2.7.4a
解决:
重建基因索引:
STAR --runThreadN 20 --runMode genomeGenerate --genomeDir ./ --genomeFastaFiles Genome.fasta --sjdbGTFfile Annotations.gtf
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