载体名称和推荐引物：多个引物可根据酶切位点选用
| 序号 | 载体名称 | 正向引物 | 反向引物 |
| 1 | pZeroBack/blunt | PJET1.2-F | PJET1.2-R |
| 2 | P3xFlag-CMV-14 | CMV-30 | CMV-24 |
| 3 | P3xFlag-CMV-7.1 | CMV-F | CMV-24 |
| 4 | P3xFlag-MYC-CMV-24 | CMV-F | CMV-24 |
| 5 | pACT | T7 | T3 |
| 6 | pACT2 | 5’AD | 3’AD |
| 7 | PACYCDUET-1(MCSI) | DuetUP1 | DuetDOWN1 |
| 8 | PACYCDUET-1(MCSII) | DuetUP2 | T7 Ter |
| 9 | pAS2-1 | 5’BD | 3’BD |
| 10 | PB42AD | PB42ADF | PB42ADR |
| 11 | pBabe-puro | pBABE5’ | pBABE3’ |
| 12 | PBACPAK8 | BAC1 | BAC2 |
| 13 | pBAD | PBAD-F | PBAD-R |
| 14 | PBIND | 5’BD | T3 |
| 15 | pBK-CMV | T7/M13-47 | T3/M13-48 |
| 16 | pBlueScript II SK(+) | M13-47 | M13-48 |
| 17 | pBluescript SK (+) | M13-47 | M13-48 |
| 18 | pBluescript SK (-) | M13-47 | M13-48 |
| 19 | pBluescript KS (+) | M13-47 | M13-48 |
| 20 | pBluescript KS (-) | M13-47 | M13-48 |
| 21 | pBV220 | PBV220+ | PBV220- |
| 22 | PCANTB5E | S1 | S6 |
| 23 | pCAT3-enhancer | pGL3+ | |
| 24 | pcDNA3.0 | pEGFP-N5/T7 | SP6/BGH |
| 25 | pcDNA3.1 | pEGFP-N5/T7 | BGH |
| 26 | pcDNA4.0 | pEGFP-N5/T7 | BGH |
| 27 | pcDNA6/V5-His | T7 | BGH |
| 28 | pcDNA6/myc-His | T7 | BGH |
| 29 | pcDNAII | T7 | SP6 |
| 30 | pCEP4 | pCMV5R/PCEP-F | pEGFP-N5/EBV-R |
| 31 | pCI-neo | T7 | T3 |
| 32 | pCMS-EGFP | T7 | T3 |
| 33 | pCMV-3Tag-4A | T3 | T7 |
| 34 | pCMV5-Flag | pCMV5F | pCMV5R |
| 35 | pCMV-MYC/NUC | pCMV5F | pCMV5R |
| 36 | pCMV-Sport | M13-20/T7 | SP6/M13-26 |
| 37 | pCMV-Tag | T3 | T7 |
| 38 | pCMV-TNT | T7/SP6 | pCMV5R |
| 39 | PCOLDI | pCold-F | pCold-R |
| 40 | pCR2.1-TOPO | M13-47 | M13-48 |
| 41 | pCR3.1 | T7 | BGH |
| 42 | pCS2 | SP6 | T7 |
| 43 | PDNR-LIB | M13-20 | M13-26 |
| 44 | pDONR/Zeo | M13-20 | M13-26 |
| 45 | pDONR221 | M13-20 | M13-26 |
| 46 | pDrive | T7 | SP6 |
| 47 | pDsRED1-C1 | pDsRED-C1-F | pECFP-C-3 |
| 48 | pDSRED2-N1 | pEGFP-N-5 | pDsRed1-N |
| 49 | pDsRed-Monomer-N1 | pEGFP-N-5 | pDsRed1-N |
| 50 | pEASY-BLUNT | M13-20 | M13-26 |
| 51 | pEASY-E1 | T7 | T7 Ter |
| 52 | pEASY-T1 | M13-47/T7 | M13-48 |
| 53 | pEASY-T3 | M13-47/T7 | M13-48/SP6 |
| 54 | pECFP-C1 | pEGFP-C-5’ | pECFP-C-3′ |
| 55 | pECFP-N1 | pEGFP-N-5’ | pEGFP-N-3’ |
| 56 | pENTR/D-TOPO | M13-20 | M13-26 |
| 57 | pET-*(His) | T7 | T7 Ter |
| 58 | PET-15B | T7 | T7 Ter |
| 59 | pET22(a,b,c) | T7 | T7 Ter |
| 60 | pET28,30,24,49 | T7 | T7 Ter |
| 61 | pET32(a,b,c) | T7/S.tag | T7 Ter |
| 62 | pET-42a (-b, -c)(+) | Stag | T7 Ter |
| 63 | pET44 | T7/s.tag | T7 Ter |
| 64 | pET50 | T7/s.tag | T7 Ter |
| 65 | PETA-IRES | EF-1aF | |
| 66 | PETDUET-1(MCSI) | DuetUP1 | DuetDOMN1 |
| 67 | PETDUET-1(MCSII) | DuetUP2 | T7 Ter |
| 68 | pEYFP-C1 | pEGFP-C5’ | pEGFP-C3′ |
| 69 | pEYFP-N1 | pEGFP-N5’ | pEGFP-N-3’ |
| 70 | pFastBac Dual (MCSI) | pFastBac-PH | pECFP-C3 |
| 71 | pFastBac HT_A,B,C | pFastBac-PH | pECFP-C3 |
| 72 | pFastBac1 | pFastBac-PH | pECFP-C3 |
| 73 | pFLAG-CMV | CMV30/pFLAG-CMV-F | CMV-24/pFLAG-CMV-R |
| 74 | pGAD424 | 5’AD | 3’AD |
| 75 | pGADGH | 5’AD | 3’AD |
| 76 | pGADT7-Rec | 5’AD/T7 | 3’AD |
| 77 | pGAPZa-A/B/C | a-FACTOR | 3’AOX |
| 78 | pGBKT7 | T7 | 3’BD |
| 79 | pGEM-T(-Easy) | M13-47 | M13-48 |
| 80 | pGEX-(*)T | pGEX-5’ | pGEX-3’ |
| 81 | PGEX-6P-1 | pGEX-5’ | pGEX-3’ |
| 82 | pGL2-Basic | GLP1 | GLP2 |
| 83 | pGL2-Control | GLP1 | GLP2 |
| 84 | Pgl3-BASIC | pGL3+ | PGL3- |
| 85 | pGL3-Enhancer | pGL3+ | PGL3- |
| 86 | PGL4.10 | RVP3 | GLP2 |
| 87 | pIRES2-EGFP | pIRES2-EGFP.P5’ | pIRES2-EGFP.P3’ |
| 88 | pJET1.2/blunt | T7/pJET1.2F | pJET1.2R |
| 89 | PLEXA | PLEXA-F | PLEXA-R |
| 90 | PLK0.1 | 人源U6 | |
| 91 | PLL3.7 | 鼠源U6 | |
| 92 | PLNCX | PLNCX-F | PLNCX-R |
| 93 | PLVX-SHRNA1 | 人源U6 | |
| 94 | PLVX-IRES-NEO | PEGFP-N5 | |
| 95 | pLXSN | pLXSN-F | pLXSN-R |
| 96 | pMAL-c2E | MalE primer | M13-47 |
| 97 | pMAL-C2x | PIRES2-EGFP.P5/PMAL-C2X-F | PIRES2-EGFP.P5/PMAL-C2X-R |
| 98 | pMAL-P2x | MalE primer | M13-47 |
| 99 | PMD18-T | M13-48 | M13-47 |
| 100 | PMD19-T | M13-47 | M13-48 |
| 101 | pmirGLO | T7 Ter | |
| 102 | pMIR-report | M13-47 | |
| 103 | pmscv | MSCV-F | MSCV-R |
| 104 | pPIC9K | 5’AOX/a-factor | 3’AOX |
| 105 | pPICZa | 5’AOX | 3’AOX |
| 106 | pPROEXHTa/b/c | M13-48 | PBV220- |
| 107 | pQE2 | pQE30+ | pQE30- |
| 108 | pQE30 | pQE30+ | pQE30- |
| 109 | pQE40 | pQE30+ | pQE30- |
| 110 | pRSET | T7 | T7 Ter |
| 111 | PRSFDUET-1(MCSI) | DuetUP1 | DuetDOWN1 |
| 112 | PRSFDUET-1(MCSII) | DuetUP2 | T7 Ter |
| 113 | pSectag(v5/His/a,b,c) | T7 | BGH |
| 114 | psi-check2 | psi-check2-F | psi-check2-R |
| 115 | pSilencer3.1-H1 hygro | M13-47 | 3.0rev |
| 116 | pSILENCEV2.0-U6 | T7 | 2.0rev |
| 117 | PsiREN-retroq | 人源U6 | |
| 118 | pSK01-T | M13-20 | M13-26 |
| 119 | pSK-CMV | T7 | T3 |
| 120 | PSP64/65 | SP6 | |
| 121 | pSP72 | T7 | SP6 |
| 122 | pSport1 | SP6 | T7 |
| 123 | pSTBlue-1 | T7 | SP6 |
| 124 | pSUPER | T7 | T3 |
| 125 | pT7Blue(R) | T7 | M13-47 |
| 126 | pTA2 | M13-47 | T3/M13-48 |
| 127 | pT-Adv | M13-47 /T7 | M13-48 |
| 128 | PTHIOHISA,B,C | TRX-FORWARD | TRX-REVERSE |
| 129 | pTO-T7 | T7 | T7 Ter |
| 130 | pTRC99a-c | PQE30+/PTRC99C-F | PBV220-/PTRC99C-R |
| 131 | pUC18(19)/118(119) | M13-47 | M13-48 |
| 132 | pUC57 | M13-26/M13-48 | M13-20/M13-47 |
| 133 | pUCm-T | M13-47/T7 | M13-48 |
| 134 | pVAX1 | T7 | BGH |
| 135 | pXT7 | T7 | SP6 |
| 136 | pYES2 | T7 | pYes2.R |
引物序列
| 引物名称 | 引物序列 |
| 27F | AGAGTTTGATCMTGGCTCAG |
| 1492R | GGTTACCTTGTTACGACTT |
| 86F | GCTCAGTAACACGTGG |
| 1340R | CGGTGTGTGCAAGGAG |
| 357F | CCTACGGGAGGCAGCAG |
| 518R | ATTACCGCGGCTGCTGG |
| 8F | AGAGTTTGATCCTGGCTCAG |
| 1541R | AAGGAGGTGATCCAGCC |
| 16F | AGAGTTTGATCCTGGCTCAG |
| 1405F | TGYACACACCGCCCGT |
| 456R | CCTTTCCCTCACGGTACTG |
| 907R | CCGTCAATTCCTTTRAGTTT |
| 341F | CTACGGGMSGCAGCAG |
| ITS1 | TCCGTAGGTGAACCTGCGG |
| ITS2 | GCTGCGTTCTTCATCGATGC |
| ITS5 | GGAAGTAAAAGTCGTAACAAGG |
| ITS4 | TCCTCCGCTTATTGATATGC |
| -96III | CCCTCATAGTTAGCGTAACG |
| 5AD | AATACCACTACAATGGATGATG |
| 3AD | GAGATGGTGCACGATGCACAGT |
| 5BD | GTGCGACATCATCATCGGAAG |
| 3BD | TAAGAGTCACTTTAAAATTTGTATAC |
| 5AOX | CTGGTTCCAATTGACAAGC |
| 3AOX | TGGCATTCTGACATCCTC |
| a-factor | TACTATTGCCAGCATTGCTGC |
| BAC1 | AACCATCTCGCAAATAAATA |
| BAC2 | ACGCACAGAATCTAGCGCTT |
| BGH | CAGGGTCAAGGAAGGCAC |
| CaMV35S-f | GCTCCTACAAATGCCATCA |
| CaMV35S-r | GATAGTGGGATTGTGCGTCA |
| PBI121(35S) | GACGCACAATCCCACTATCC |
| CMV-F | CGCAAATGGGCGGTAGGCGTG |
| CMV-R | TCGTTGGGCGGTCAGC |
| CMV-24 | TATTAGGACAAGGCTGGTGGGCAC |
| CMV-30 | AATGTCGTAATAACCCCGCCCCGTTGACGC |
| C-MYCREV | CTCTTCTGAGATGAGTTTTTG |
| Colidown | ATCAGCCTAGGAACGCCCAAC |
| CYC1 | GCGTGAATGTAAGCGTGAC |
| DuetDOWN1 | GATTATGCGGCCGTGTACAA |
| DuetUP1 | GATCTCGACGCTCTCCCT |
| DuetUP2 | TTGTACACGGCCGCATAATC |
| EBV-R | GTGGTTTGTCCAAACTCATC |
| GAL4AD | TACCACTACAATGGATG |
| GAL4BD | TCATCGGAAGAGAGTAG |
| GLp1 | TGTATCTTATGGTACTGTAACTG |
| GLp2 | CTTTATGTTTTTGGCGTCTTCCA |
| GPD-promoter-F | CGGTAGGTATTGATTGTAATTCTG |
| H1 | TCGCTATGTGTTCTGGGAAA |
| M13+ | AGGGTTTTCCCAGTCACG |
| M13-47 | AGGGTTTTCCCAGTCACG |
| M13- | GAGCGGATAACAATTTCACAC |
| M13-48 | GAGCGGATAACAATTTCACAC |
| M13F | GTTGTAAAACGACGGCCAG |
| M13-20 | GTTGTAAAACGACGGCCAG |
| M13R | CAGGAAACAGCTATGAC |
| M13-26 | CAGGAAACAGCTATGAC |
| M13-40 | GTCGTGGACTGGGAAAAC |
| M13-D | AGGGTTTTCCCAGTCACG |
| RV-M | GAGCGGATAACAATTTCACAC |
| malefor | GGTCGTCAGACTGTCGATGAAGCC |
| MSCV-F | CCCTTGAACCTCCTCGTTCGACC |
| PMSCV-R | GAGACGTGCTACTTCCATTTGTC |
| N26FOR | CATCATAACGGTTCTGGC |
| NL1 | GCATATCAATAAGCGGAGGAAAAG |
| NL4 | GGTCCGTGTTTCAAGACGG |
| NS1 | GTAGTCATATGCTTGTCTC |
| NS6 | GCATCACAGACCTGTTATTGCCTC |
| NS8 | TCCGCAGGTTCACCTACGGA |
| P1 | CCAGGCTTTACACTTTATGC |
| P2 | GCGATTAAGTTGGGTAACGC |
| P5 | TGCGTACTGCGGTGATCAAC |
| P3 | CTGCAAGGCGATTAAGTTGG |
| P10-PROFOR | CGGACCTTTAATTCAACCC |
| PACGP67RV | TTGGTTCTTGCCGGGTC |
| pADTrack-F | GGGCCATTTACCGTAAGT |
| PAS2-1F | TCATCGGAAGCGAGTAG |
| PAS2-1R | CGTTTTAAAACCTAAGAGTCAC |
| PB42ADF | CCAGCCTCTTGCTGAGTGGAGATG |
| PB42ADR | AAGCCGACAACCTTGATTGGAG |
| PBABE3 | ACCCTAACTGACACACATTCC |
| pBABE5 | CTTTATCCAGCCCTCAC |
| PBAD-F | ATGCCATAGCATTTTTATCC |
| PBAD-R | ATCTGTATCAGGCTGAAAATC |
| PBRrevBam | GGTGATGTCGGCGATATAGG |
| PBV220+ | GGGCAGCATTCAAAGCAG |
| PBV220- | TTATCAGACCGCTTCTGC |
| PCDNA3.0F-F2 | CTCTGGCTAACTAGAGAACC |
| PCDNA3.0R-R2 | GGCAACTAGAAGGCACAGTC |
| PCDNA3.1F | CTAGAGAACCCACTGCTTAC |
| PCDNA3.1R | TAGAAGGCACAGTCGAGG |
| PCEP-F | AGAGCTCGTTTAGTGAACCG |
| pCMV5-F | TCTAAAAGCTGCGGAATTGT |
| pCMV5-R | TCCAAACTCATCAATGTATC |
| PDC316-F | ACGTGGGTATAAGAGGCG |
| PDC316-R | CGATGCTAGACGATCCAG |
| pDSRED-C1-F | AGCTGGACATCACCTCCCACAACG |
| pDsRed1-N | GTACTGGAACTGGGGGGACAG |
| pDonr-For | CTGGCAGTTCCCTACTCTCG |
| pDonr-Rev | TGTAACATCAGAGATTTTGAGACAC |
| PEF-F | TCAAGCCTCAGACAGTGGTTC |
| pEGFP-N5 | CGGTGGGAGGTCTATATAAG |
| pEGFP-N3 | GTCGCCGTCCAGCTCGACCAG |
| pEGFP-C5 | CATGGTCCTGCTGGAGTTCGT |
| pEGFP-C3 | TATGTTTCAGGTTCAGGG |
| PETBlueDOWN | ATAGCTTTAATGCGGTAGTT |
| pETUpstream | ATGCGTCCGGCGTAGA |
| pFastBac-F | TGAAGTGGTTCGCATCCTC |
| pFastBac-R | TGGACAAACCACAACTAGAATG |
| pFastBac-PH | AAATGATAACCATCTCGC |
| pGAPforward | GTCCCTATTTCAATCAATTGAA |
| pGEX5 | GCAAGCCACGTTTGGTG |
| PGEX3 | GAGCTGCATGTGTCAGAGG |
| pGL3+ | TAGCAAAATAGGCTGTCCC |
| pGL3- | CTTTATGTTTTTGGCGTCTTC |
| PinPoint-Primer | CGTGACGCGGTGCAGGGCG |
| PIRES2-EGFP.P5 | GTAGGCGTGTACGGTGGGAG |
| PIRES2-EGFP.P3 | AACGCACACCGGCCTTATTC |
| pFlag-CMV-F | GGTAGGCGTGTACGGTGG |
| pFlag-CMV-R | GCACTGGAGTGGCAACTT |
| pJET1.2F | CGACTCACTATAGGGAGAGCGGC |
| pJET1.2R | AAGAACATCGATTTTCCATGGCAG |
| pLEXA-F | CGTCAGCAGAGCTTCACCAT |
| pLEXA-R | TAAAACCTAAGAGTCACTTT |
| pLNCX-F | AGCTCGTTTAGTGAACCGTCAGATCG |
| pLNCX-R | ACCTACAGGTGGGGTCTTTCATTCCC |
| pLVX-R | ACTTGTGGCCATTCACAT |
| PLXSN-5 | CCCTTGAACCTCCTCGTTCGACC |
| PLXSN-3 | GAGCCTGGGGACTTTCCACACCC |
| PPC86-F | TATAACGCGTTTGGAATCACT |
| PPC86-R | GTAAATTTCTGGCAAGGTAGAC |
| pQE30+ | GTGAGCGGATAACAATTTCAC |
| pQE30- | CTGAACAAATCCAGATGGAG |
| pRNA-U6Forward | TACGATACAAGGCTGTTAGAGAG |
| psi-check2-F | ATGGGTAAGTACATCAAGAG |
| psi-check2-R | GAGGTCCGAAGACTCATTTA |
| PSilencer4.1-F | AGGCGATTAAGTTGGGTA |
| PSilencer4.1-R | CGGTAGGCGTGTACGGTG |
| pShuttle-CMV-F | GGTCTATATAAGCAGAGCTG |
| pShuttle-CMV-R | GTGGTATGGCTGATTATGATCAG |
| PT5 | TCCGAGATCTGGACGAGC |
| pTarget.F | CCAGGATTTTCCCAGTCAC |
| pTarget.R | GGCTTTACACTTTATGCTTC |
| PTARGET-SEQ | TTACGCCAAGTTATTTAGGTGACA |
| PTRC99C-F | TTGCGCCGACATCATAAC |
| PTRC99C-R | CTGCGTTCTGATTTAATCTG |
| PTRCHISA-F | CATGGTATGGCTAGCATGAC |
| PTRCHISA-R | CAGGCTGAAAATCTTCTCTC |
| RFP-Nrev | GTTCACGGTGCCCTCC |
| RV3 | CTAGCAAAATAGGCTGTCCC |
| RV4 | GACGATAGTCATGCCCCGCG |
| S1 | CAACGTGAAAAAATTATTATTCGC |
| S6 | GTAAATGAATTTTCTGTATGAGG |
| SP6 | ATTTAGGTGACACTATAGAA |
| S-TAG | CGAACGCCAGCACATGGACA |
| SV40 | GGAGGCTTTTTTGGAGGC |
| T7 | TAATACGACTCACTATAGG |
| T3 | ATTAACCCTCACTAAAGG |
| T7-TER | GCTAGTTATTGCTCAGCGG |
| TRX-FORWARD | TTCCTCGACGCTAACCTG |
| TRX-REVERSE | TGTAAAACGACGGCCAGTGC |
| TRX-F | CATATGAGCGATAAAATTATTCAC |
| TRX-R | GGATCCCTTGTCATCGTCATCACCA |
| U6-renyuan | ATGGACTATCATATGCTTACCGTA |
| U6-shuyuan | CAGTGCAGGGGAAAGAATAGTAGAC |
| U6-PROFOR | CCGTAACTTGAAAGTATTTCG |
| 2.0revprimer | AGGCGATTAAGTTGGGTA |
| 3.0rev | GAGTTAGCTCACTCATTAGGC |
| 5TriplEx2 | CTCCGAGATCTGGACGAGC |
| 17BASE | ACATCCACTTTGCCTTTCTC |
| Amp-F | TGAGATCCAGTTCGATGTAA |
| Amp-R | TCAGGCAACTATGGATGAAC |
| Kan-F | ATTCTCACCGGATTCAGT |
| Kan-R | GTCTGACCATCTCATCTGTA |
| pCold-F | GTAAAGCACGCCATATCGC |
| pCold-R | CCAAATGGCAGGGATCTTAG |
| PA | AGAGTTTGATCCTGGCTCAG |
| PB | AGGAGGTGATCCAGCCGC |
| PLL3.7-F | AACTATAGAGGCTTAATGTG |
| puc57-F | GCCATTCGCCATTCAGG |
| puc57-R | TAGCTCACTCATTAGGCAC |
| Kan-P1 | AACTCATCGAGCATCAAATG |
| Kan-P2 | CTCTAGGCCGCGATTAAATTC |
| 3C-forward | CTTCCTGTTAGTTAGTTACTTAAGC |
| 3creverse | GTAACATCAGAGATTTTGAGACAC |
| PCAG-F | GCAACGTGCTGGTTATTGTG |
| PTRCHISA-RV | ATCTGTATCAGGCTGAAAATC |
| PBAD-RV | ATCTGTATCAGGCTGAAAATC |
| PYES2-R | GCGTGAATGTAAGCGTGAC |
| ITS3 | GCATCGATGAAGAACGCAGC |
| V5reverse | ACCGAGGAGAGGGTTAGGGAT |
| L14724 | GACTTGAAAAACCACCGTTG |
| H15915 | CTCCGATCTCCGGATTACAAGAC |
| EF-1aF | TCAAGCCTCAGACAGTGGTTC |
| PLKO.1-5 | GACTATCATATGCTTACCGT |
| Lco1490 | GGTCAACAAATCATAAAGATATTGG |
| Hco2198 | TAAACTTCAGGGTGACCAAAAAATCA |
| WPRE-R | CATAGCGTAAAAGGAGCAACA |
| LR0R | GTACCCGCTGAACTTAAGC |
| LR5 | ATCCTGAGGGAAACTTC |
| PCAGGS-F | ATGTTCATGCCTTCTTCTTT |
| PCAGGS-R | TTCCTTTATTAGCCAGAAGT |
| YSFF | GAGCCAGTGAGTTGATTGTGT |
| YSRR | AGGCTTTTGACTTTCTGCTATGGAC |
| SV40pA-R | GAAATTTGTGATGCTATTGC |
| pENTR-F | CTACAAACTCTTCCTGTTAGTTAG |
| pENTR-R | ATGGCTCATAACACCCCTTG |
| IRES-R | CCTCACATTGCCAAAAGACG |
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