常用载体测序引物选择和序列

载体名称和推荐引物:多个引物可根据酶切位点选用

序号 载体名称 正向引物 反向引物
1 pZeroBack/blunt PJET1.2-F PJET1.2-R
2 P3xFlag-CMV-14 CMV-30 CMV-24
3 P3xFlag-CMV-7.1 CMV-F CMV-24
4 P3xFlag-MYC-CMV-24 CMV-F CMV-24
5 pACT T7 T3
6 pACT2 5’AD 3’AD
7 PACYCDUET-1(MCSI) DuetUP1 DuetDOWN1
8 PACYCDUET-1(MCSII) DuetUP2 T7 Ter
9 pAS2-1 5’BD 3’BD
10 PB42AD PB42ADF PB42ADR
11 pBabe-puro pBABE5’ pBABE3’
12 PBACPAK8 BAC1 BAC2
13 pBAD PBAD-F PBAD-R
14 PBIND 5’BD T3
15 pBK-CMV T7/M13-47 T3/M13-48
16 pBlueScript II SK(+) M13-47 M13-48
17 pBluescript SK (+) M13-47 M13-48
18 pBluescript SK (-) M13-47 M13-48
19 pBluescript KS (+) M13-47 M13-48
20 pBluescript KS (-) M13-47 M13-48
21 pBV220 PBV220+ PBV220-
22 PCANTB5E S1 S6
23 pCAT3-enhancer pGL3+
24 pcDNA3.0 pEGFP-N5/T7 SP6/BGH
25 pcDNA3.1 pEGFP-N5/T7 BGH
26 pcDNA4.0 pEGFP-N5/T7 BGH
27 pcDNA6/V5-His  T7 BGH
28 pcDNA6/myc-His  T7 BGH
29 pcDNAII T7 SP6
30 pCEP4 pCMV5R/PCEP-F pEGFP-N5/EBV-R
31 pCI-neo T7 T3
32 pCMS-EGFP T7 T3
33 pCMV-3Tag-4A T3 T7
34 pCMV5-Flag pCMV5F pCMV5R
35 pCMV-MYC/NUC pCMV5F pCMV5R
36 pCMV-Sport M13-20/T7 SP6/M13-26
37 pCMV-Tag T3 T7
38 pCMV-TNT T7/SP6 pCMV5R
39 PCOLDI pCold-F pCold-R
40 pCR2.1-TOPO M13-47 M13-48
41 pCR3.1 T7 BGH
42 pCS2 SP6 T7
43 PDNR-LIB M13-20 M13-26
44 pDONR/Zeo M13-20 M13-26
45 pDONR221 M13-20 M13-26
46 pDrive T7 SP6
47 pDsRED1-C1 pDsRED-C1-F pECFP-C-3
48 pDSRED2-N1  pEGFP-N-5 pDsRed1-N
49 pDsRed-Monomer-N1 pEGFP-N-5 pDsRed1-N
50 pEASY-BLUNT M13-20 M13-26
51 pEASY-E1 T7 T7 Ter
52 pEASY-T1 M13-47/T7 M13-48
53 pEASY-T3 M13-47/T7 M13-48/SP6
54 pECFP-C1 pEGFP-C-5’ pECFP-C-3′ 
55 pECFP-N1 pEGFP-N-5’ pEGFP-N-3’
56 pENTR/D-TOPO M13-20 M13-26
57 pET-*(His) T7 T7 Ter
58 PET-15B T7 T7 Ter
59 pET22(a,b,c) T7 T7 Ter
60 pET28,30,24,49 T7 T7 Ter
61 pET32(a,b,c) T7/S.tag T7 Ter
62 pET-42a (-b, -c)(+) Stag T7 Ter
63 pET44 T7/s.tag T7 Ter
64 pET50 T7/s.tag T7 Ter
65 PETA-IRES EF-1aF
66 PETDUET-1(MCSI) DuetUP1 DuetDOMN1
67 PETDUET-1(MCSII) DuetUP2 T7 Ter
68 pEYFP-C1 pEGFP-C5’ pEGFP-C3′ 
69 pEYFP-N1 pEGFP-N5’ pEGFP-N-3’
70 pFastBac Dual (MCSI) pFastBac-PH pECFP-C3
71 pFastBac HT_A,B,C pFastBac-PH pECFP-C3
72 pFastBac1 pFastBac-PH pECFP-C3
73 pFLAG-CMV  CMV30/pFLAG-CMV-F CMV-24/pFLAG-CMV-R
74 pGAD424  5’AD 3’AD
75 pGADGH  5’AD 3’AD
76 pGADT7-Rec 5’AD/T7 3’AD
77 pGAPZa-A/B/C a-FACTOR  3’AOX
78 pGBKT7 T7 3’BD
79 pGEM-T(-Easy) M13-47 M13-48
80 pGEX-(*)T pGEX-5’ pGEX-3’
81 PGEX-6P-1 pGEX-5’ pGEX-3’
82 pGL2-Basic GLP1 GLP2
83 pGL2-Control GLP1 GLP2
84 Pgl3-BASIC pGL3+ PGL3-
85 pGL3-Enhancer pGL3+ PGL3-
86 PGL4.10 RVP3 GLP2
87 pIRES2-EGFP pIRES2-EGFP.P5’ pIRES2-EGFP.P3’
88 pJET1.2/blunt T7/pJET1.2F pJET1.2R
89 PLEXA PLEXA-F PLEXA-R
90 PLK0.1 人源U6 
91 PLL3.7 鼠源U6
92 PLNCX PLNCX-F PLNCX-R
93 PLVX-SHRNA1 人源U6 
94 PLVX-IRES-NEO  PEGFP-N5
95 pLXSN  pLXSN-F pLXSN-R
96 pMAL-c2E MalE primer M13-47
97 pMAL-C2x PIRES2-EGFP.P5/PMAL-C2X-F PIRES2-EGFP.P5/PMAL-C2X-R
98 pMAL-P2x MalE primer M13-47
99 PMD18-T M13-48 M13-47
100 PMD19-T M13-47 M13-48
101 pmirGLO T7 Ter
102 pMIR-report M13-47
103 pmscv MSCV-F MSCV-R
104 pPIC9K 5’AOX/a-factor 3’AOX
105 pPICZa 5’AOX 3’AOX
106 pPROEXHTa/b/c M13-48 PBV220-
107 pQE2 pQE30+ pQE30-
108 pQE30 pQE30+ pQE30-
109 pQE40 pQE30+ pQE30-
110 pRSET T7 T7 Ter
111 PRSFDUET-1(MCSI) DuetUP1 DuetDOWN1
112 PRSFDUET-1(MCSII) DuetUP2 T7 Ter
113 pSectag(v5/His/a,b,c)  T7 BGH
114 psi-check2 psi-check2-F psi-check2-R
115 pSilencer3.1-H1 hygro M13-47 3.0rev
116 pSILENCEV2.0-U6 T7 2.0rev
117 PsiREN-retroq 人源U6
118 pSK01-T M13-20 M13-26
119 pSK-CMV T7 T3
120 PSP64/65 SP6
121 pSP72 T7 SP6
122 pSport1 SP6 T7
123 pSTBlue-1 T7 SP6
124 pSUPER T7 T3
125 pT7Blue(R) T7 M13-47
126 pTA2  M13-47 T3/M13-48
127 pT-Adv M13-47 /T7 M13-48
128 PTHIOHISA,B,C TRX-FORWARD TRX-REVERSE
129 pTO-T7 T7 T7 Ter
130 pTRC99a-c PQE30+/PTRC99C-F PBV220-/PTRC99C-R
131 pUC18(19)/118(119) M13-47 M13-48
132 pUC57 M13-26/M13-48 M13-20/M13-47
133 pUCm-T M13-47/T7 M13-48
134 pVAX1 T7 BGH
135 pXT7  T7 SP6
136 pYES2  T7 pYes2.R

 

引物序列

引物名称 引物序列
27F AGAGTTTGATCMTGGCTCAG
1492R GGTTACCTTGTTACGACTT
86F GCTCAGTAACACGTGG
1340R CGGTGTGTGCAAGGAG
357F CCTACGGGAGGCAGCAG
518R ATTACCGCGGCTGCTGG
8F AGAGTTTGATCCTGGCTCAG
1541R AAGGAGGTGATCCAGCC
16F AGAGTTTGATCCTGGCTCAG
1405F TGYACACACCGCCCGT
456R CCTTTCCCTCACGGTACTG
907R CCGTCAATTCCTTTRAGTTT
341F CTACGGGMSGCAGCAG
ITS1 TCCGTAGGTGAACCTGCGG
ITS2 GCTGCGTTCTTCATCGATGC
ITS5 GGAAGTAAAAGTCGTAACAAGG
ITS4 TCCTCCGCTTATTGATATGC
-96III CCCTCATAGTTAGCGTAACG
5AD AATACCACTACAATGGATGATG
3AD GAGATGGTGCACGATGCACAGT
5BD GTGCGACATCATCATCGGAAG
3BD TAAGAGTCACTTTAAAATTTGTATAC
5AOX CTGGTTCCAATTGACAAGC
3AOX TGGCATTCTGACATCCTC
a-factor TACTATTGCCAGCATTGCTGC
BAC1 AACCATCTCGCAAATAAATA
BAC2 ACGCACAGAATCTAGCGCTT
BGH CAGGGTCAAGGAAGGCAC
CaMV35S-f GCTCCTACAAATGCCATCA
CaMV35S-r GATAGTGGGATTGTGCGTCA
PBI121(35S) GACGCACAATCCCACTATCC
CMV-F CGCAAATGGGCGGTAGGCGTG
CMV-R TCGTTGGGCGGTCAGC
CMV-24 TATTAGGACAAGGCTGGTGGGCAC
CMV-30 AATGTCGTAATAACCCCGCCCCGTTGACGC
C-MYCREV CTCTTCTGAGATGAGTTTTTG
Colidown ATCAGCCTAGGAACGCCCAAC
CYC1 GCGTGAATGTAAGCGTGAC
DuetDOWN1 GATTATGCGGCCGTGTACAA
DuetUP1 GATCTCGACGCTCTCCCT
DuetUP2 TTGTACACGGCCGCATAATC
EBV-R GTGGTTTGTCCAAACTCATC
GAL4AD TACCACTACAATGGATG
GAL4BD TCATCGGAAGAGAGTAG
GLp1 TGTATCTTATGGTACTGTAACTG
GLp2 CTTTATGTTTTTGGCGTCTTCCA
GPD-promoter-F CGGTAGGTATTGATTGTAATTCTG
H1 TCGCTATGTGTTCTGGGAAA
M13+ AGGGTTTTCCCAGTCACG
M13-47 AGGGTTTTCCCAGTCACG
M13- GAGCGGATAACAATTTCACAC
M13-48 GAGCGGATAACAATTTCACAC
M13F GTTGTAAAACGACGGCCAG
M13-20 GTTGTAAAACGACGGCCAG
M13R CAGGAAACAGCTATGAC
M13-26 CAGGAAACAGCTATGAC
M13-40 GTCGTGGACTGGGAAAAC
M13-D AGGGTTTTCCCAGTCACG
RV-M GAGCGGATAACAATTTCACAC
malefor GGTCGTCAGACTGTCGATGAAGCC
MSCV-F CCCTTGAACCTCCTCGTTCGACC
PMSCV-R GAGACGTGCTACTTCCATTTGTC
N26FOR CATCATAACGGTTCTGGC
NL1 GCATATCAATAAGCGGAGGAAAAG
NL4 GGTCCGTGTTTCAAGACGG
NS1 GTAGTCATATGCTTGTCTC
NS6 GCATCACAGACCTGTTATTGCCTC
NS8 TCCGCAGGTTCACCTACGGA
P1 CCAGGCTTTACACTTTATGC
P2 GCGATTAAGTTGGGTAACGC
P5 TGCGTACTGCGGTGATCAAC
P3 CTGCAAGGCGATTAAGTTGG
P10-PROFOR CGGACCTTTAATTCAACCC
PACGP67RV TTGGTTCTTGCCGGGTC
pADTrack-F GGGCCATTTACCGTAAGT
PAS2-1F TCATCGGAAGCGAGTAG
PAS2-1R CGTTTTAAAACCTAAGAGTCAC
PB42ADF CCAGCCTCTTGCTGAGTGGAGATG
PB42ADR AAGCCGACAACCTTGATTGGAG
PBABE3 ACCCTAACTGACACACATTCC
pBABE5 CTTTATCCAGCCCTCAC
PBAD-F ATGCCATAGCATTTTTATCC
PBAD-R ATCTGTATCAGGCTGAAAATC
PBRrevBam GGTGATGTCGGCGATATAGG
PBV220+ GGGCAGCATTCAAAGCAG
PBV220- TTATCAGACCGCTTCTGC
PCDNA3.0F-F2 CTCTGGCTAACTAGAGAACC
PCDNA3.0R-R2 GGCAACTAGAAGGCACAGTC
PCDNA3.1F CTAGAGAACCCACTGCTTAC
PCDNA3.1R TAGAAGGCACAGTCGAGG
PCEP-F AGAGCTCGTTTAGTGAACCG
pCMV5-F TCTAAAAGCTGCGGAATTGT
pCMV5-R TCCAAACTCATCAATGTATC
PDC316-F ACGTGGGTATAAGAGGCG
PDC316-R CGATGCTAGACGATCCAG
pDSRED-C1-F AGCTGGACATCACCTCCCACAACG
pDsRed1-N GTACTGGAACTGGGGGGACAG
pDonr-For CTGGCAGTTCCCTACTCTCG
pDonr-Rev TGTAACATCAGAGATTTTGAGACAC
PEF-F TCAAGCCTCAGACAGTGGTTC
pEGFP-N5 CGGTGGGAGGTCTATATAAG
pEGFP-N3 GTCGCCGTCCAGCTCGACCAG
pEGFP-C5 CATGGTCCTGCTGGAGTTCGT
pEGFP-C3 TATGTTTCAGGTTCAGGG
PETBlueDOWN ATAGCTTTAATGCGGTAGTT
pETUpstream ATGCGTCCGGCGTAGA
pFastBac-F TGAAGTGGTTCGCATCCTC
pFastBac-R TGGACAAACCACAACTAGAATG
pFastBac-PH AAATGATAACCATCTCGC
pGAPforward GTCCCTATTTCAATCAATTGAA
pGEX5 GCAAGCCACGTTTGGTG
PGEX3 GAGCTGCATGTGTCAGAGG
pGL3+ TAGCAAAATAGGCTGTCCC
pGL3- CTTTATGTTTTTGGCGTCTTC
PinPoint-Primer CGTGACGCGGTGCAGGGCG
PIRES2-EGFP.P5 GTAGGCGTGTACGGTGGGAG
PIRES2-EGFP.P3 AACGCACACCGGCCTTATTC
pFlag-CMV-F GGTAGGCGTGTACGGTGG
pFlag-CMV-R GCACTGGAGTGGCAACTT
pJET1.2F CGACTCACTATAGGGAGAGCGGC
pJET1.2R AAGAACATCGATTTTCCATGGCAG
pLEXA-F CGTCAGCAGAGCTTCACCAT
pLEXA-R TAAAACCTAAGAGTCACTTT
pLNCX-F AGCTCGTTTAGTGAACCGTCAGATCG
pLNCX-R ACCTACAGGTGGGGTCTTTCATTCCC
pLVX-R ACTTGTGGCCATTCACAT
PLXSN-5 CCCTTGAACCTCCTCGTTCGACC
PLXSN-3 GAGCCTGGGGACTTTCCACACCC
PPC86-F TATAACGCGTTTGGAATCACT
PPC86-R GTAAATTTCTGGCAAGGTAGAC
pQE30+ GTGAGCGGATAACAATTTCAC
pQE30- CTGAACAAATCCAGATGGAG
pRNA-U6Forward TACGATACAAGGCTGTTAGAGAG
psi-check2-F ATGGGTAAGTACATCAAGAG
psi-check2-R GAGGTCCGAAGACTCATTTA
PSilencer4.1-F AGGCGATTAAGTTGGGTA
PSilencer4.1-R CGGTAGGCGTGTACGGTG
pShuttle-CMV-F GGTCTATATAAGCAGAGCTG
pShuttle-CMV-R GTGGTATGGCTGATTATGATCAG
PT5 TCCGAGATCTGGACGAGC
pTarget.F CCAGGATTTTCCCAGTCAC
pTarget.R GGCTTTACACTTTATGCTTC
PTARGET-SEQ TTACGCCAAGTTATTTAGGTGACA
PTRC99C-F TTGCGCCGACATCATAAC
PTRC99C-R CTGCGTTCTGATTTAATCTG
PTRCHISA-F CATGGTATGGCTAGCATGAC
PTRCHISA-R CAGGCTGAAAATCTTCTCTC
RFP-Nrev GTTCACGGTGCCCTCC
RV3 CTAGCAAAATAGGCTGTCCC
RV4 GACGATAGTCATGCCCCGCG
S1 CAACGTGAAAAAATTATTATTCGC
S6 GTAAATGAATTTTCTGTATGAGG
SP6 ATTTAGGTGACACTATAGAA
S-TAG CGAACGCCAGCACATGGACA
SV40 GGAGGCTTTTTTGGAGGC
T7 TAATACGACTCACTATAGG
T3 ATTAACCCTCACTAAAGG
T7-TER GCTAGTTATTGCTCAGCGG
TRX-FORWARD TTCCTCGACGCTAACCTG
TRX-REVERSE TGTAAAACGACGGCCAGTGC
TRX-F CATATGAGCGATAAAATTATTCAC
TRX-R GGATCCCTTGTCATCGTCATCACCA
U6-renyuan ATGGACTATCATATGCTTACCGTA
U6-shuyuan CAGTGCAGGGGAAAGAATAGTAGAC
U6-PROFOR CCGTAACTTGAAAGTATTTCG
2.0revprimer AGGCGATTAAGTTGGGTA
3.0rev GAGTTAGCTCACTCATTAGGC
5TriplEx2 CTCCGAGATCTGGACGAGC
17BASE ACATCCACTTTGCCTTTCTC
Amp-F TGAGATCCAGTTCGATGTAA
Amp-R TCAGGCAACTATGGATGAAC
Kan-F ATTCTCACCGGATTCAGT
Kan-R GTCTGACCATCTCATCTGTA
pCold-F GTAAAGCACGCCATATCGC
pCold-R CCAAATGGCAGGGATCTTAG
PA AGAGTTTGATCCTGGCTCAG
PB AGGAGGTGATCCAGCCGC
PLL3.7-F AACTATAGAGGCTTAATGTG
puc57-F GCCATTCGCCATTCAGG
puc57-R TAGCTCACTCATTAGGCAC
Kan-P1 AACTCATCGAGCATCAAATG
Kan-P2 CTCTAGGCCGCGATTAAATTC
3C-forward CTTCCTGTTAGTTAGTTACTTAAGC
3creverse GTAACATCAGAGATTTTGAGACAC
PCAG-F GCAACGTGCTGGTTATTGTG
PTRCHISA-RV ATCTGTATCAGGCTGAAAATC
PBAD-RV ATCTGTATCAGGCTGAAAATC
PYES2-R GCGTGAATGTAAGCGTGAC
ITS3 GCATCGATGAAGAACGCAGC
V5reverse ACCGAGGAGAGGGTTAGGGAT
L14724 GACTTGAAAAACCACCGTTG
H15915 CTCCGATCTCCGGATTACAAGAC
EF-1aF TCAAGCCTCAGACAGTGGTTC
PLKO.1-5 GACTATCATATGCTTACCGT
Lco1490 GGTCAACAAATCATAAAGATATTGG
Hco2198 TAAACTTCAGGGTGACCAAAAAATCA
WPRE-R CATAGCGTAAAAGGAGCAACA
LR0R GTACCCGCTGAACTTAAGC
LR5 ATCCTGAGGGAAACTTC
PCAGGS-F ATGTTCATGCCTTCTTCTTT
PCAGGS-R TTCCTTTATTAGCCAGAAGT
YSFF GAGCCAGTGAGTTGATTGTGT
YSRR AGGCTTTTGACTTTCTGCTATGGAC
SV40pA-R GAAATTTGTGATGCTATTGC
pENTR-F CTACAAACTCTTCCTGTTAGTTAG
pENTR-R ATGGCTCATAACACCCCTTG
IRES-R CCTCACATTGCCAAAAGACG

 

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