载体名称和推荐引物：多个引物可根据酶切位点选用

序号	载体名称	正向引物	反向引物
1	pZeroBack/blunt	PJET1.2-F	PJET1.2-R
2	P3xFlag-CMV-14	CMV-30	CMV-24
3	P3xFlag-CMV-7.1	CMV-F	CMV-24
4	P3xFlag-MYC-CMV-24	CMV-F	CMV-24
5	pACT	T7	T3
6	pACT2	5’AD	3’AD
7	PACYCDUET-1(MCSI)	DuetUP1	DuetDOWN1
8	PACYCDUET-1(MCSII)	DuetUP2	T7 Ter
9	pAS2-1	5’BD	3’BD
10	PB42AD	PB42ADF	PB42ADR
11	pBabe-puro	pBABE5’	pBABE3’
12	PBACPAK8	BAC1	BAC2
13	pBAD	PBAD-F	PBAD-R
14	PBIND	5’BD	T3
15	pBK-CMV	T7/M13-47	T3/M13-48
16	pBlueScript II SK(+)	M13-47	M13-48
17	pBluescript SK (+)	M13-47	M13-48
18	pBluescript SK (-)	M13-47	M13-48
19	pBluescript KS (+)	M13-47	M13-48
20	pBluescript KS (-)	M13-47	M13-48
21	pBV220	PBV220+	PBV220-
22	PCANTB5E	S1	S6
23	pCAT3-enhancer	pGL3+
24	pcDNA3.0	pEGFP-N5/T7	SP6/BGH
25	pcDNA3.1	pEGFP-N5/T7	BGH
26	pcDNA4.0	pEGFP-N5/T7	BGH
27	pcDNA6/V5-His	T7	BGH
28	pcDNA6/myc-His	T7	BGH
29	pcDNAII	T7	SP6
30	pCEP4	pCMV5R/PCEP-F	pEGFP-N5/EBV-R
31	pCI-neo	T7	T3
32	pCMS-EGFP	T7	T3
33	pCMV-3Tag-4A	T3	T7
34	pCMV5-Flag	pCMV5F	pCMV5R
35	pCMV-MYC/NUC	pCMV5F	pCMV5R
36	pCMV-Sport	M13-20/T7	SP6/M13-26
37	pCMV-Tag	T3	T7
38	pCMV-TNT	T7/SP6	pCMV5R
39	PCOLDI	pCold-F	pCold-R
40	pCR2.1-TOPO	M13-47	M13-48
41	pCR3.1	T7	BGH
42	pCS2	SP6	T7
43	PDNR-LIB	M13-20	M13-26
44	pDONR/Zeo	M13-20	M13-26
45	pDONR221	M13-20	M13-26
46	pDrive	T7	SP6
47	pDsRED1-C1	pDsRED-C1-F	pECFP-C-3
48	pDSRED2-N1	pEGFP-N-5	pDsRed1-N
49	pDsRed-Monomer-N1	pEGFP-N-5	pDsRed1-N
50	pEASY-BLUNT	M13-20	M13-26
51	pEASY-E1	T7	T7 Ter
52	pEASY-T1	M13-47/T7	M13-48
53	pEASY-T3	M13-47/T7	M13-48/SP6
54	pECFP-C1	pEGFP-C-5’	pECFP-C-3′
55	pECFP-N1	pEGFP-N-5’	pEGFP-N-3’
56	pENTR/D-TOPO	M13-20	M13-26
57	pET-*(His)	T7	T7 Ter
58	PET-15B	T7	T7 Ter
59	pET22(a,b,c)	T7	T7 Ter
60	pET28,30,24,49	T7	T7 Ter
61	pET32(a,b,c)	T7/S.tag	T7 Ter
62	pET-42a (-b, -c)(+)	Stag	T7 Ter
63	pET44	T7/s.tag	T7 Ter
64	pET50	T7/s.tag	T7 Ter
65	PETA-IRES	EF-1aF
66	PETDUET-1(MCSI)	DuetUP1	DuetDOMN1
67	PETDUET-1(MCSII)	DuetUP2	T7 Ter
68	pEYFP-C1	pEGFP-C5’	pEGFP-C3′
69	pEYFP-N1	pEGFP-N5’	pEGFP-N-3’
70	pFastBac Dual (MCSI)	pFastBac-PH	pECFP-C3
71	pFastBac HT_A,B,C	pFastBac-PH	pECFP-C3
72	pFastBac1	pFastBac-PH	pECFP-C3
73	pFLAG-CMV	CMV30/pFLAG-CMV-F	CMV-24/pFLAG-CMV-R
74	pGAD424	5’AD	3’AD
75	pGADGH	5’AD	3’AD
76	pGADT7-Rec	5’AD/T7	3’AD
77	pGAPZa-A/B/C	a-FACTOR	3’AOX
78	pGBKT7	T7	3’BD
79	pGEM-T(-Easy)	M13-47	M13-48
80	pGEX-(*)T	pGEX-5’	pGEX-3’
81	PGEX-6P-1	pGEX-5’	pGEX-3’
82	pGL2-Basic	GLP1	GLP2
83	pGL2-Control	GLP1	GLP2
84	Pgl3-BASIC	pGL3+	PGL3-
85	pGL3-Enhancer	pGL3+	PGL3-
86	PGL4.10	RVP3	GLP2
87	pIRES2-EGFP	pIRES2-EGFP.P5’	pIRES2-EGFP.P3’
88	pJET1.2/blunt	T7/pJET1.2F	pJET1.2R
89	PLEXA	PLEXA-F	PLEXA-R
90	PLK0.1	人源U6
91	PLL3.7	鼠源U6
92	PLNCX	PLNCX-F	PLNCX-R
93	PLVX-SHRNA1	人源U6
94	PLVX-IRES-NEO	PEGFP-N5
95	pLXSN	pLXSN-F	pLXSN-R
96	pMAL-c2E	MalE primer	M13-47
97	pMAL-C2x	PIRES2-EGFP.P5/PMAL-C2X-F	PIRES2-EGFP.P5/PMAL-C2X-R
98	pMAL-P2x	MalE primer	M13-47
99	PMD18-T	M13-48	M13-47
100	PMD19-T	M13-47	M13-48
101	pmirGLO		T7 Ter
102	pMIR-report	M13-47
103	pmscv	MSCV-F	MSCV-R
104	pPIC9K	5’AOX/a-factor	3’AOX
105	pPICZa	5’AOX	3’AOX
106	pPROEXHTa/b/c	M13-48	PBV220-
107	pQE2	pQE30+	pQE30-
108	pQE30	pQE30+	pQE30-
109	pQE40	pQE30+	pQE30-
110	pRSET	T7	T7 Ter
111	PRSFDUET-1(MCSI)	DuetUP1	DuetDOWN1
112	PRSFDUET-1(MCSII)	DuetUP2	T7 Ter
113	pSectag(v5/His/a,b,c)	T7	BGH
114	psi-check2	psi-check2-F	psi-check2-R
115	pSilencer3.1-H1 hygro	M13-47	3.0rev
116	pSILENCEV2.0-U6	T7	2.0rev
117	PsiREN-retroq	人源U6
118	pSK01-T	M13-20	M13-26
119	pSK-CMV	T7	T3
120	PSP64/65	SP6
121	pSP72	T7	SP6
122	pSport1	SP6	T7
123	pSTBlue-1	T7	SP6
124	pSUPER	T7	T3
125	pT7Blue(R)	T7	M13-47
126	pTA2	M13-47	T3/M13-48
127	pT-Adv	M13-47 /T7	M13-48
128	PTHIOHISA,B,C	TRX-FORWARD	TRX-REVERSE
129	pTO-T7	T7	T7 Ter
130	pTRC99a-c	PQE30+/PTRC99C-F	PBV220-/PTRC99C-R
131	pUC18(19)/118(119)	M13-47	M13-48
132	pUC57	M13-26/M13-48	M13-20/M13-47
133	pUCm-T	M13-47/T7	M13-48
134	pVAX1	T7	BGH
135	pXT7	T7	SP6
136	pYES2	T7	pYes2.R

引物序列

引物名称	引物序列
27F	AGAGTTTGATCMTGGCTCAG
1492R	GGTTACCTTGTTACGACTT
86F	GCTCAGTAACACGTGG
1340R	CGGTGTGTGCAAGGAG
357F	CCTACGGGAGGCAGCAG
518R	ATTACCGCGGCTGCTGG
8F	AGAGTTTGATCCTGGCTCAG
1541R	AAGGAGGTGATCCAGCC
16F	AGAGTTTGATCCTGGCTCAG
1405F	TGYACACACCGCCCGT
456R	CCTTTCCCTCACGGTACTG
907R	CCGTCAATTCCTTTRAGTTT
341F	CTACGGGMSGCAGCAG
ITS1	TCCGTAGGTGAACCTGCGG
ITS2	GCTGCGTTCTTCATCGATGC
ITS5	GGAAGTAAAAGTCGTAACAAGG
ITS4	TCCTCCGCTTATTGATATGC
-96III	CCCTCATAGTTAGCGTAACG
5AD	AATACCACTACAATGGATGATG
3AD	GAGATGGTGCACGATGCACAGT
5BD	GTGCGACATCATCATCGGAAG
3BD	TAAGAGTCACTTTAAAATTTGTATAC
5AOX	CTGGTTCCAATTGACAAGC
3AOX	TGGCATTCTGACATCCTC
a-factor	TACTATTGCCAGCATTGCTGC
BAC1	AACCATCTCGCAAATAAATA
BAC2	ACGCACAGAATCTAGCGCTT
BGH	CAGGGTCAAGGAAGGCAC
CaMV35S-f	GCTCCTACAAATGCCATCA
CaMV35S-r	GATAGTGGGATTGTGCGTCA
PBI121(35S)	GACGCACAATCCCACTATCC
CMV-F	CGCAAATGGGCGGTAGGCGTG
CMV-R	TCGTTGGGCGGTCAGC
CMV-24	TATTAGGACAAGGCTGGTGGGCAC
CMV-30	AATGTCGTAATAACCCCGCCCCGTTGACGC
C-MYCREV	CTCTTCTGAGATGAGTTTTTG
Colidown	ATCAGCCTAGGAACGCCCAAC
CYC1	GCGTGAATGTAAGCGTGAC
DuetDOWN1	GATTATGCGGCCGTGTACAA
DuetUP1	GATCTCGACGCTCTCCCT
DuetUP2	TTGTACACGGCCGCATAATC
EBV-R	GTGGTTTGTCCAAACTCATC
GAL4AD	TACCACTACAATGGATG
GAL4BD	TCATCGGAAGAGAGTAG
GLp1	TGTATCTTATGGTACTGTAACTG
GLp2	CTTTATGTTTTTGGCGTCTTCCA
GPD-promoter-F	CGGTAGGTATTGATTGTAATTCTG
H1	TCGCTATGTGTTCTGGGAAA
M13+	AGGGTTTTCCCAGTCACG
M13-47	AGGGTTTTCCCAGTCACG
M13-	GAGCGGATAACAATTTCACAC
M13-48	GAGCGGATAACAATTTCACAC
M13F	GTTGTAAAACGACGGCCAG
M13-20	GTTGTAAAACGACGGCCAG
M13R	CAGGAAACAGCTATGAC
M13-26	CAGGAAACAGCTATGAC
M13-40	GTCGTGGACTGGGAAAAC
M13-D	AGGGTTTTCCCAGTCACG
RV-M	GAGCGGATAACAATTTCACAC
malefor	GGTCGTCAGACTGTCGATGAAGCC
MSCV-F	CCCTTGAACCTCCTCGTTCGACC
PMSCV-R	GAGACGTGCTACTTCCATTTGTC
N26FOR	CATCATAACGGTTCTGGC
NL1	GCATATCAATAAGCGGAGGAAAAG
NL4	GGTCCGTGTTTCAAGACGG
NS1	GTAGTCATATGCTTGTCTC
NS6	GCATCACAGACCTGTTATTGCCTC
NS8	TCCGCAGGTTCACCTACGGA
P1	CCAGGCTTTACACTTTATGC
P2	GCGATTAAGTTGGGTAACGC
P5	TGCGTACTGCGGTGATCAAC
P3	CTGCAAGGCGATTAAGTTGG
P10-PROFOR	CGGACCTTTAATTCAACCC
PACGP67RV	TTGGTTCTTGCCGGGTC
pADTrack-F	GGGCCATTTACCGTAAGT
PAS2-1F	TCATCGGAAGCGAGTAG
PAS2-1R	CGTTTTAAAACCTAAGAGTCAC
PB42ADF	CCAGCCTCTTGCTGAGTGGAGATG
PB42ADR	AAGCCGACAACCTTGATTGGAG
PBABE3	ACCCTAACTGACACACATTCC
pBABE5	CTTTATCCAGCCCTCAC
PBAD-F	ATGCCATAGCATTTTTATCC
PBAD-R	ATCTGTATCAGGCTGAAAATC
PBRrevBam	GGTGATGTCGGCGATATAGG
PBV220+	GGGCAGCATTCAAAGCAG
PBV220-	TTATCAGACCGCTTCTGC
PCDNA3.0F-F2	CTCTGGCTAACTAGAGAACC
PCDNA3.0R-R2	GGCAACTAGAAGGCACAGTC
PCDNA3.1F	CTAGAGAACCCACTGCTTAC
PCDNA3.1R	TAGAAGGCACAGTCGAGG
PCEP-F	AGAGCTCGTTTAGTGAACCG
pCMV5-F	TCTAAAAGCTGCGGAATTGT
pCMV5-R	TCCAAACTCATCAATGTATC
PDC316-F	ACGTGGGTATAAGAGGCG
PDC316-R	CGATGCTAGACGATCCAG
pDSRED-C1-F	AGCTGGACATCACCTCCCACAACG
pDsRed1-N	GTACTGGAACTGGGGGGACAG
pDonr-For	CTGGCAGTTCCCTACTCTCG
pDonr-Rev	TGTAACATCAGAGATTTTGAGACAC
PEF-F	TCAAGCCTCAGACAGTGGTTC
pEGFP-N5	CGGTGGGAGGTCTATATAAG
pEGFP-N3	GTCGCCGTCCAGCTCGACCAG
pEGFP-C5	CATGGTCCTGCTGGAGTTCGT
pEGFP-C3	TATGTTTCAGGTTCAGGG
PETBlueDOWN	ATAGCTTTAATGCGGTAGTT
pETUpstream	ATGCGTCCGGCGTAGA
pFastBac-F	TGAAGTGGTTCGCATCCTC
pFastBac-R	TGGACAAACCACAACTAGAATG
pFastBac-PH	AAATGATAACCATCTCGC
pGAPforward	GTCCCTATTTCAATCAATTGAA
pGEX5	GCAAGCCACGTTTGGTG
PGEX3	GAGCTGCATGTGTCAGAGG
pGL3+	TAGCAAAATAGGCTGTCCC
pGL3-	CTTTATGTTTTTGGCGTCTTC
PinPoint-Primer	CGTGACGCGGTGCAGGGCG
PIRES2-EGFP.P5	GTAGGCGTGTACGGTGGGAG
PIRES2-EGFP.P3	AACGCACACCGGCCTTATTC
pFlag-CMV-F	GGTAGGCGTGTACGGTGG
pFlag-CMV-R	GCACTGGAGTGGCAACTT
pJET1.2F	CGACTCACTATAGGGAGAGCGGC
pJET1.2R	AAGAACATCGATTTTCCATGGCAG
pLEXA-F	CGTCAGCAGAGCTTCACCAT
pLEXA-R	TAAAACCTAAGAGTCACTTT
pLNCX-F	AGCTCGTTTAGTGAACCGTCAGATCG
pLNCX-R	ACCTACAGGTGGGGTCTTTCATTCCC
pLVX-R	ACTTGTGGCCATTCACAT
PLXSN-5	CCCTTGAACCTCCTCGTTCGACC
PLXSN-3	GAGCCTGGGGACTTTCCACACCC
PPC86-F	TATAACGCGTTTGGAATCACT
PPC86-R	GTAAATTTCTGGCAAGGTAGAC
pQE30+	GTGAGCGGATAACAATTTCAC
pQE30-	CTGAACAAATCCAGATGGAG
pRNA-U6Forward	TACGATACAAGGCTGTTAGAGAG
psi-check2-F	ATGGGTAAGTACATCAAGAG
psi-check2-R	GAGGTCCGAAGACTCATTTA
PSilencer4.1-F	AGGCGATTAAGTTGGGTA
PSilencer4.1-R	CGGTAGGCGTGTACGGTG
pShuttle-CMV-F	GGTCTATATAAGCAGAGCTG
pShuttle-CMV-R	GTGGTATGGCTGATTATGATCAG
PT5	TCCGAGATCTGGACGAGC
pTarget.F	CCAGGATTTTCCCAGTCAC
pTarget.R	GGCTTTACACTTTATGCTTC
PTARGET-SEQ	TTACGCCAAGTTATTTAGGTGACA
PTRC99C-F	TTGCGCCGACATCATAAC
PTRC99C-R	CTGCGTTCTGATTTAATCTG
PTRCHISA-F	CATGGTATGGCTAGCATGAC
PTRCHISA-R	CAGGCTGAAAATCTTCTCTC
RFP-Nrev	GTTCACGGTGCCCTCC
RV3	CTAGCAAAATAGGCTGTCCC
RV4	GACGATAGTCATGCCCCGCG
S1	CAACGTGAAAAAATTATTATTCGC
S6	GTAAATGAATTTTCTGTATGAGG
SP6	ATTTAGGTGACACTATAGAA
S-TAG	CGAACGCCAGCACATGGACA
SV40	GGAGGCTTTTTTGGAGGC
T7	TAATACGACTCACTATAGG
T3	ATTAACCCTCACTAAAGG
T7-TER	GCTAGTTATTGCTCAGCGG
TRX-FORWARD	TTCCTCGACGCTAACCTG
TRX-REVERSE	TGTAAAACGACGGCCAGTGC
TRX-F	CATATGAGCGATAAAATTATTCAC
TRX-R	GGATCCCTTGTCATCGTCATCACCA
U6-renyuan	ATGGACTATCATATGCTTACCGTA
U6-shuyuan	CAGTGCAGGGGAAAGAATAGTAGAC
U6-PROFOR	CCGTAACTTGAAAGTATTTCG
2.0revprimer	AGGCGATTAAGTTGGGTA
3.0rev	GAGTTAGCTCACTCATTAGGC
5TriplEx2	CTCCGAGATCTGGACGAGC
17BASE	ACATCCACTTTGCCTTTCTC
Amp-F	TGAGATCCAGTTCGATGTAA
Amp-R	TCAGGCAACTATGGATGAAC
Kan-F	ATTCTCACCGGATTCAGT
Kan-R	GTCTGACCATCTCATCTGTA
pCold-F	GTAAAGCACGCCATATCGC
pCold-R	CCAAATGGCAGGGATCTTAG
PA	AGAGTTTGATCCTGGCTCAG
PB	AGGAGGTGATCCAGCCGC
PLL3.7-F	AACTATAGAGGCTTAATGTG
puc57-F	GCCATTCGCCATTCAGG
puc57-R	TAGCTCACTCATTAGGCAC
Kan-P1	AACTCATCGAGCATCAAATG
Kan-P2	CTCTAGGCCGCGATTAAATTC
3C-forward	CTTCCTGTTAGTTAGTTACTTAAGC
3creverse	GTAACATCAGAGATTTTGAGACAC
PCAG-F	GCAACGTGCTGGTTATTGTG
PTRCHISA-RV	ATCTGTATCAGGCTGAAAATC
PBAD-RV	ATCTGTATCAGGCTGAAAATC
PYES2-R	GCGTGAATGTAAGCGTGAC
ITS3	GCATCGATGAAGAACGCAGC
V5reverse	ACCGAGGAGAGGGTTAGGGAT
L14724	GACTTGAAAAACCACCGTTG
H15915	CTCCGATCTCCGGATTACAAGAC
EF-1aF	TCAAGCCTCAGACAGTGGTTC
PLKO.1-5	GACTATCATATGCTTACCGT
Lco1490	GGTCAACAAATCATAAAGATATTGG
Hco2198	TAAACTTCAGGGTGACCAAAAAATCA
WPRE-R	CATAGCGTAAAAGGAGCAACA
LR0R	GTACCCGCTGAACTTAAGC
LR5	ATCCTGAGGGAAACTTC
PCAGGS-F	ATGTTCATGCCTTCTTCTTT
PCAGGS-R	TTCCTTTATTAGCCAGAAGT
YSFF	GAGCCAGTGAGTTGATTGTGT
YSRR	AGGCTTTTGACTTTCTGCTATGGAC
SV40pA-R	GAAATTTGTGATGCTATTGC
pENTR-F	CTACAAACTCTTCCTGTTAGTTAG
pENTR-R	ATGGCTCATAACACCCCTTG
IRES-R	CCTCACATTGCCAAAAGACG