大肠杆菌中的常见的基因突变-Gene mutations in E.coli strains

Gene(s) Description Functional Consequence
dam DNA adenine methylase mutation (GATC) Preparing unmethylated DNA, important when trying to cut with certain restriction enzymes (ex: ClaI or XbaI)
dcm DNA cytosine methylase mutation (CCWGG) Preparing unmethylated DNA, important when trying to cut with certain restriction enzymes that are methylation sensitive.
dnaJ Mutation in a chaperonin gene Increases the stability of certain expressed proteins
endA, endA1 Endonuclease I (nonspecific cleavage of dsDNA ) mutation Improves plasmid yield
F Host does (F’) or does not (F-) contain the fertility plasmid. A low copy-number plasmid, encodes proteins needed for bacterial conjugation. Genes listed on F´ are wild-type unless indicated otherwise
fhuA (formerly tonA) ferric hydroxamate uptake, iron uptake receptor mutation. T1/T5 Phage resistance
gal Mutation in galactose metabolism pathway Cells cannot grow on galactose only
gyrA, gyrA96 DNA gyrase mutation Confers resistance to nalidixic acid
 hsdRMS hsdR(rk-, mk+) Unmethylated DNA not degraded, cell still can methylate DNA
hsdS(rk-,mk-) Unmethylated DNA not degraded, cell cannot methylate DNA
lac Lac operon mutations Blue/white screening of clones
lacIq lac repressor overproduced, expression from pLac repressed more
LacZ β-galactosidase activity abolished
lacY Lactose permease inactivated, lactose cannot be taken up by cell
mcrA, mcrBC Inactivation of pathway that cleaves methylated cytosine DNA Allows for uptake of foreign (methylated) DNA
mrr, Δ(mcrC-mrr) Inactivation of pathway that cleaves methylated adenine or cytosine DNA Allows for uptake of foreign (methylated) DNA
recA, recA1, recA13 Mutation in a DNA-dependent ATPase that is essential for recombination and general DNA repair Reduces plasmid recombination, increases plasmid stability
recBCD Exonuclease V activity abolished Increased plasmid stability, reduced recombination
relA or relA1 Relaxed phenotype, mutation eliminating stringent factor Allows RNA synthesis in absence of protein synthesis
Ptrc-ccdA Propagation of ccdB-containing plasmids
Hte High transformation efficiency
deoR constitutive expression of genes for deoxyribose synthesis Allows uptake of large plasmids
hee High electroporation efficiency
supE44 (glnV44) Suppression of the amber (UAG) stop codon by glutamine insertion
supF (tyrT) Suppression of the amber (UAG) stop codon by tyrosine insertion
λ-thi-1 or thi1 Mutation in thiamine metabolism Requires exogenous thiamine for growth
ara disruption of arabinose metabolism pathway Inability to utilize arabinose as a carbon source
leuB β-isopropyl malate dehydrogenase inactivated Requires exogenous leucine source for growth
proAB mutation in proline biosynthesis pathway Requires exogenous proline source for growth
rpsL Mutation in subunit S12 of 30S ribosome Confers resistance to streptomycin
Tn10 Confers resistance to tetracycline

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