| 细胞株名称 | Natural resistance | Primary Use | 基因型/Genotype |
| ccdB Survival 2 T1R | TOP10 derivative. For propagating plasmids expressing the ccdB gene (important in Gateway cloning). | F–mcrA Δ(mrr-hsdRMS-mcrBC) Φ80lacZΔM15 ΔlacX74 recA1 araΔ139 Δ(ara-leu)7697 galU galK rpsL (StrR) endA1 nupG fhuA::IS2 | |
| DB3.1 | Streptomycin | HB101 derivative. For propagating plasmids expressing the ccdB gene (important in Gateway cloning). | F- gyrA462 endA1 glnV44 Δ(sr1-recA) mcrB mrr hsdS20(rB–, mB–) ara14 galK2 lacY1 proA2 rpsL20(Smr) xyl5 Δleu mtl1 |
| DH10B | Streptomycin | MC1061 derivative. General cloning and storage, blue/white screening, leucine auxotroph. | F– endA1 recA1 galE15 galK16 nupG rpsL ΔlacX74 Φ80lacZΔM15 araD139 Δ(ara,leu)7697 mcrA Δ(mrr-hsdRMS-mcrBC) λ– |
| DH5alpha | General cloning and storage of common plasmids, blue/white screening. | F– endA1 glnV44 thi-1 recA1 relA1 gyrA96 deoR nupG Φ80dlacZΔM15 Δ(lacZYA-argF)U169, hsdR17(rK– mK+), λ– | |
| HB101 | Streptomycin | Hybrid of E. coli K12 and E. coli B (mostly K12, though), common lab strain for cloning and storage of pBR322 plasmids. | F– mcrB mrr hsdS20(rB– mB–) recA13 leuB6 ara-14 proA2 lacY1 galK2 xyl-5 mtl-1 rpsL20(SmR) glnV44 λ– |
| JM109 | General cloning and plasmid maintenance, blue/white screening, partly restriction-deficient; good strain for cloning repetitive DNA. | endA1 glnV44 thi-1 relA1 gyrA96 recA1 mcrB+ Δ(lac-proAB) e14- [F’ traD36 proAB+ lacIq lacZΔM15] hsdR17(rK–mK+) | |
| JM110 | Streptomycin | For storing plasmids that should not be Dam or Dcm methylated, allows for methylation sensitive restriction enzymes to cut the plasmid after preparation. | rpsL thr leu thi lacY galK galT ara tonA tsx dam dcm glnV44 Δ(lac-proAB) e14- [F’ traD36 proAB+ lacIq lacZΔM15] hsdR17(rK–mK+) |
| MC1061 | Streptomycin | Parent of DH10B/TOP10 and derived strains, common lab cloning and storage strain. | F– Δ(ara-leu)7697 [araD139]B/r Δ(codB-lacI)3 galK16 galE15 λ– e14– mcrA0 relA1 rpsL150(strR) spoT1 mcrB1 hsdR2(r–m+) |
| MG1655 | Wild type K-12 strain; first published sequence of an E. coli K-12 strain. | F– λ– ilvG- rfb-50 rph-1 | |
| NEB Stable | For cloning into and storage of lentiviral and retroviral vectors or cloning or repeated sequences with the potential to recombine. | F’ proA+B+ lacIq ∆(lacZ)M15 zzf::Tn10 (TetR) ∆(ara-leu) 7697 araD139 fhuA ∆lacX74 galK16 galE15 e14- Φ80dlacZ∆M15 recA1 relA1 endA1 nupG rpsL (StrR) rph spoT1 ∆(mrr-hsdRMS-mcrBC) | |
| Pir1 | For cloning and maintenance of a plasmids with R6Kγ origin; contains a mutant allele of the pir gene that maintains the plasmids at ~250 copies per cell. | F- ∆lac169 rpoS(Am) robA1 creC510 hsdR514 endA recA1 uidA(∆MluI)::pir-116 | |
| Stbl2 | JM109-derived. For cloning into and storage of lentiviral and retroviral vectors or cloning or repeated sequences with the potential to recombine. | F- endA1 glnV44 thi-1 recA1 gyrA96 relA1 Δ(lac-proAB) mcrA Δ(mcrBC-hsdRMS-mrr) λ– | |
| Stbl3 | Streptomycin | Derived from HB101. For cloning into and storage of lentiviral and retroviral vectors or cloning or repeated sequences with the potential to recombine. This strain is endA+, so column-based DNA preps require an additional wash step. | F- glnV44 recA13 mcrB mrr hsdS20(rB-, mB-) ara-14 galK2 lacY1 proA2 rpsL20 xyl-5 leu mtl-1 |
| Stbl4 | Electrocompetent cells for cloning and storage of unstable DNA and lentiviral/retroviral vectors. Used by Addgene for pooled library amplification. | F’ mcrA Δ(mcrBC-hsdRMS-mrr) recA1 endA1 gyrA96 gal–thi-1 supE44 λ–relA1 Δ(lac-proAB)/F’ proAB+lacIqZΔM15 Tn10 (TetR) | |
| TOP10 | Streptomycin | MC1061 derivative. General cloning and storage, blue/white screening. | F- mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74 nupG recA1 araD139 Δ(ara-leu)7697 galE15 galK16 rpsL(StrR) endA1 λ– |
| XL1 Blue | Tetracycline | Blue/white screening and routine cloning, nalidixic acid resistant. | endA1 gyrA96(nalR) thi-1 recA1 relA1 lac glnV44 F'[ ::Tn10 proAB+ lacIq Δ(lacZ)M15] hsdR17(rK– mK+) |
| XL10 Gold | Tetracycline and Chloramphenicol | High competency cloning and propagation of large plasmids, ligated DNA, and libraries, nalidixic acid resistant. | endA1 glnV44 recA1 thi-1 gyrA96 relA1 lac Hte Δ(mcrA)183 Δ(mcrCB-hsdSMR-mrr)173 tetR F'[proAB lacIqZΔM15 Tn10(TetR Amy CmR)] |
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