单细胞测序
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R:integrating and analysis single-cell datasets
Introduction In this notebook I will go over several integration techniques for single-cell -omics data. These technique…
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Cellranger分析10X Genomics single-cell RNA-seq data单细胞原始数据
cellranger是处理从 10X Genomics 平台生成的单细胞 RNA-seq 数据的命令行工具。cellranger 由 10X Genomics 构建，专门用于处理从其平台生成的数据，然而有几种开源工具可以与 cellrang…
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R: Load in data from 10X
Description Enables easy loading of sparse data matrices provided by 10X genomics. Usage Read10X( data.dir, gene.column …
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VISION：Functional Interpretation for scRNA-seq Data
Preliminaries If you have yet to install VISION, we recommend installing the package from Github to install this package…
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Developmental cell:整合单细胞蛋白质组学和转录组学的人类乳腺图谱-A human breast atlas integrating single-cell proteomics and transcriptomics
Published:May 25, 2022DOI:https://doi.org/10.1016/j.devcel.2022.05.003 The breast is a dynamic organ whose response to p…
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在Ubuntu 18.04上编译bcl2fastq v2.20
与MiSeq使用MiSeq Reporter自动将二进制碱基调用（BCL）文件转换为FASTQ格式不同，NextSeq、HiSeq和NovaSeq系统的输出需要用户开发的或第三方的数据分析工具，如bcl2fastq，来转换为FASTQ。除了…
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conda 安装R语言及其R
1.配置Conda镜像源 conda config –add channels https://mirrors.tuna.tsinghua.edu.cn/anaconda/pkgs/free conda config –add chan…
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Cell Ranger – 6.1.2安装和使用
1.安装curl/wget curl -o cellranger-6.1.2.tar.gz “https://cf.10xgenomics.com/releases/cell-exp/cellranger-6.1.2.tar.gz?Expi…
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从小鼠皮肤伤口分离巨噬细胞用于单细胞RNA测序
了解组织修复中的巨噬细胞异质性是一个重大挑战。在这里，我们描述了一个协议，它结合了从皮肤伤口中分离免疫细胞和随后基于流式细胞仪的伤口巨噬细胞分拣和单细胞RNA测序。我们使用原始Smart-seq2协议的修改版来提高速度和准确性。该协议对于分…
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Smart-seq3 Protocol
• The current protocols is based on the TN5 from Nextera Xt kit. However Illumina TDE1, works as well. • For determini…
