gRNA Design在线设计网站资源

    • Green Listed – a CRISPR Screen ToolDesign gRNAs for custom CRISPR screens targeting a long or short list of genes provided by the user.
    • Eukaryotic Pathogen CRISPR guide RNA/DNA Design Tool (EuPaGDT)gRNA design tool with extensive selection of eukaryotic pathogen genomes (200+) that can predict gRNA targets in gene families, HDR oligonucleotide design, and batch processing for designing genome-wide gRNA libraries. PubMed PMID 28348817.
    • CRISPORThis tool helps design (10 different prediction scores), clone (primer design), and evaluate gRNAs, as well as predict off-targets, for CRISPR in 180+ genomes. PubMed PMID: 27380939.
    • sgRNA Scorer 2.0
      From the Church Lab: a tool that identifies putative target sites for S. pyogenes Cas9, S. thermophilus Cas9, or Cpf from your input sequence or list of sequences.
    • RGEN (RNA-guided endonuclease) Tools: Cas-OFFinder & Cas-Designer
      From the Kim Lab, Cas-OFFinder identifies gRNA target sequences from an input sequence and checks for off-target binding. Currently supports: Drosophila, Arabidopsis, zebrafish, C. elegans, mouse, human, rat, cow, dog, pig, Thale cress, rice (Oryza sativa), tomato, corn, monkey (macaca mulatta).Cas-Designer searches for targets that maximize knockout efficiency while having a a low probability of off-target effects. Cas-Designer integrates information from the Kim Lab’s Cas-OFFinder and Microhomology predictor.
    • CRISPR-ERA: CRISPR-mediated editing, repression, and activationFrom the Qi Lab, a sgRNA design tool for genome editing, as well as gene regulation (repression and activation). Genome support for bacteria (E. coli, B. subtilis), yeast (S. cerevisiae), worm (C. elegans), fruit fly, zebrafish, mouse, rat, and human.
    • CCTop – CRISPR/Cas9 target online predictorIdentifies candidate sgRNA target sites by off-target quality. Validated for gene inactivation, NHEJ, and HDR. Reference genomes include Arabidopsis, C. elegans , sea squirt, cavefish, Chinese hamster, fruit fly, human, rice fish, mouse, silk worm, stickleback, tobacco, tomato, frog (X. laevis and X. tropicalis), and zebrafish.
    • Off-Spotter: tool for CRISPR/Cas designProgram for designing optimal gRNAs. Provides feedback on number of potential off-targets, target’s genomic location, and genome annotation. Available genomes are human (hg19 & hg38), mouse (mm10), and yeast (strain w303).
    • CRISPR MultiTargeter
      Can be used to identify novel gRNA target sites in a single gene, as well as a target site common to a set of similar sequences. Organisms include human, mouse, rat, chicken, frog, zebrafish, fly, worm, Japanese rice fish, maize, Arabidopsis, and rice. Proof-of-concept performed in zebrafish.

 

    • ZiFiT Targeter
      Originally developed to identify zinc finger nuclease sites, this tool from the Joung Lab has been expanded to identify potential DNA target sites for TALEs and CRISPR/Cas.

 

    • CRISPRdirect
      From the Database Center for Life Science (DBCLS) in Japan; Identify candidate gRNA target sequences in an input sequence, which can be an accession number, genomic location, pasted nucleotide sequence, or a sequence text file you upload. Currently supports: Human, mouse, rat, marmoset, pig, chicken, frog (X. tropicalis and X. laevis), zebrafish, sea squirt, Drosophila, C. elegans, Arabidopsis, rice, sorghum, silkworm, and budding and fission yeast.

 

    • Target Finder (Feng Zhang lab)
      Identifies gRNA target sequences from an input sequence and checks for off-target binding. Currently supports: Drosophila, Arabidopsis, zebrafish, C. elegans, mouse, human, rat, rabbit, pig, possum, chicken, dog, mosquito, and stickleback.

 

    • E-CRISP
      Identifies gRNA target sequences from an input sequence and checks for off-target binding. Currently supports: Drosophila, Arabidopsis, zebrafish, C. elegans , mouse, human, rat, yeast, frog, Brachypodium distachyon, Oryza sativa, Oryzias latipes

 

如若转载,请注明出处:https://www.ouq.net/1993.html

(0)
打赏 微信打赏,为服务器增加50M流量 微信打赏,为服务器增加50M流量 支付宝打赏,为服务器增加50M流量 支付宝打赏,为服务器增加50M流量
上一篇 07/27/2022 22:01
下一篇 07/27/2022

相关推荐

  • Star Protocol: 从宏基因组中提取 CRISPR 靶向序列

    基于同源性的搜索通常用于从宏基因组中发现移动遗传元件 (MGE),但它在很大程度上依赖于数据库中的参考基因组。 该方法可以在不使用参考数据库的情况下从组装的人宏基因组序列中提取 CRISPR 靶向序列。 我们描述了宏基因组重叠群的组装、CR…

    08/18/2022
    369
  • CRISPResso 深度测序数据分析CRISPR Cas9基因组编辑效果

    CRISPResso是一个强大且易于使用的计算流程,能够准确量化和可视化CRISPR-Cas9的结果,以及全面评估其对编码序列、功能性非编码元素和非靶点的影响。该算法允许对非同源末端连接(NHEJ)和同源定向修复(HDR)频率进行量化。 C…

    07/27/2022
    636
  • CRISPR敲入同源臂的理想长度是多少?

    基因敲除物(<2 kb)的同源臂的理想长度是多少?长的基因敲除物(>5 kb)的同源臂的理想长度是多少? 在果蝇中,长度为0.5-2 kb的同源臂可以很容易地用于敲除。在蚊子中,0.5-1 kb的同源臂适用于敲除<2 kb…

    CRISPR基因编辑 04/25/2022
    587