copGFP标记物是一种来自桡足类动物（Pontellina sp.）的新型天然绿色单体GFP样蛋白。copGFP蛋白是一种无毒、无聚集的蛋白，具有快速的蛋白成熟度，在广泛的pH值（pH4-12）范围内具有高稳定性，并且不需要任何额外的辅助因子或底物。copGFP蛋白具有非常明亮的荧光，至少超过广泛使用的Aequorea victoria GFP突变体EGFP亮度的1.3倍。copGFP蛋白发出的绿色荧光具有以下特点：
发射波长emission wavelength max – 502 nm;
激发波长excitation wavelength max – 482 nm;
quantum yield – 0.6;
extinction coefficient – 70,000 M-1 cm-1
Due to its exceptional properties, copGFP is an excellent fluorescent marker which can be used instead of EGFP。
| CHARACTERISTIC | |
|---|---|
| * Brightness is a product of extinction coefficient and quantum yield, divided by 1000. | |
| Molecular weight, kDa | 26 |
| Fluorescence color | green |
| Excitation maximum, nm | 482 |
| Emission maximum, nm | 502 |
| Quantum yield | 0.60 |
| Extinction coefficient, M-1cm-1 | 70000 |
| Brightness* | 42.0 |
| Brightness, % of EGFP | 126 |
| pKa | 4.3 |
| Structure | tetramer |
| Aggregation | yes |
| Maturation rate at 37°C | super fast |
| Photostability | high |
| Cell toxicity | not observed |
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