本文列出一代测序常用的通用引物，一般由测序公司免费提供。
| 引物名称 | 引物序列 |
| 27F | AGAGTTTGATCMTGGCTCAG |
| 1492R | GGTTACCTTGTTACGACTT |
| 86F | GCTCAGTAACACGTGG |
| 1340R | CGGTGTGTGCAAGGAG |
| 357F | CCTACGGGAGGCAGCAG |
| 518R | ATTACCGCGGCTGCTGG |
| 8F | AGAGTTTGATCCTGGCTCAG |
| 1541R | AAGGAGGTGATCCAGCC |
| 16F | AGAGTTTGATCCTGGCTCAG |
| 1405F | TGYACACACCGCCCGT |
| 456R | CCTTTCCCTCACGGTACTG |
| 907R | CCGTCAATTCCTTTRAGTTT |
| 341F | CTACGGGMSGCAGCAG |
| ITS1 | TCCGTAGGTGAACCTGCGG |
| ITS2 | GCTGCGTTCTTCATCGATGC |
| ITS5 | GGAAGTAAAAGTCGTAACAAGG |
| ITS4 | TCCTCCGCTTATTGATATGC |
| -96III | CCCTCATAGTTAGCGTAACG |
| 5AD | AATACCACTACAATGGATGATG |
| 3AD | GAGATGGTGCACGATGCACAGT |
| 5BD | GTGCGACATCATCATCGGAAG |
| 3BD | TAAGAGTCACTTTAAAATTTGTATAC |
| 5AOX | CTGGTTCCAATTGACAAGC |
| 3AOX | TGGCATTCTGACATCCTC |
| a-factor | TACTATTGCCAGCATTGCTGC |
| BAC1 | AACCATCTCGCAAATAAATA |
| BAC2 | ACGCACAGAATCTAGCGCTT |
| BGH | CAGGGTCAAGGAAGGCAC |
| CaMV35S-f | GCTCCTACAAATGCCATCA |
| CaMV35S-r | GATAGTGGGATTGTGCGTCA |
| PBI121(35S) | GACGCACAATCCCACTATCC |
| CMV-F | CGCAAATGGGCGGTAGGCGTG |
| CMV-R | TCGTTGGGCGGTCAGC |
| CMV-24 | TATTAGGACAAGGCTGGTGGGCAC |
| CMV-30 | AATGTCGTAATAACCCCGCCCCGTTGACGC |
| C-MYCREV | CTCTTCTGAGATGAGTTTTTG |
| Colidown | ATCAGCCTAGGAACGCCCAAC |
| CYC1 | GCGTGAATGTAAGCGTGAC |
| DuetDOWN1 | GATTATGCGGCCGTGTACAA |
| DuetUP1 | GATCTCGACGCTCTCCCT |
| DuetUP2 | TTGTACACGGCCGCATAATC |
| EBV-R | GTGGTTTGTCCAAACTCATC |
| GAL4AD | TACCACTACAATGGATG |
| GAL4BD | TCATCGGAAGAGAGTAG |
| GLp1 | TGTATCTTATGGTACTGTAACTG |
| GLp2 | CTTTATGTTTTTGGCGTCTTCCA |
| GPD-promoter-F | CGGTAGGTATTGATTGTAATTCTG |
| H1 | TCGCTATGTGTTCTGGGAAA |
| M13+ | AGGGTTTTCCCAGTCACG |
| M13-47 | AGGGTTTTCCCAGTCACG |
| M13- | GAGCGGATAACAATTTCACAC |
| M13-48 | GAGCGGATAACAATTTCACAC |
| M13F | GTTGTAAAACGACGGCCAG |
| M13-20 | GTTGTAAAACGACGGCCAG |
| M13R | CAGGAAACAGCTATGAC |
| M13-26 | CAGGAAACAGCTATGAC |
| M13-40 | GTCGTGGACTGGGAAAAC |
| M13-D | AGGGTTTTCCCAGTCACG |
| RV-M | GAGCGGATAACAATTTCACAC |
| malefor | GGTCGTCAGACTGTCGATGAAGCC |
| MSCV-F | CCCTTGAACCTCCTCGTTCGACC |
| PMSCV-R | GAGACGTGCTACTTCCATTTGTC |
| N26FOR | CATCATAACGGTTCTGGC |
| NL1 | GCATATCAATAAGCGGAGGAAAAG |
| NL4 | GGTCCGTGTTTCAAGACGG |
| NS1 | GTAGTCATATGCTTGTCTC |
| NS6 | GCATCACAGACCTGTTATTGCCTC |
| NS8 | TCCGCAGGTTCACCTACGGA |
| P1 | CCAGGCTTTACACTTTATGC |
| P2 | GCGATTAAGTTGGGTAACGC |
| P5 | TGCGTACTGCGGTGATCAAC |
| P3 | CTGCAAGGCGATTAAGTTGG |
| P10-PROFOR | CGGACCTTTAATTCAACCC |
| PACGP67RV | TTGGTTCTTGCCGGGTC |
| pADTrack-F | GGGCCATTTACCGTAAGT |
| PAS2-1F | TCATCGGAAGCGAGTAG |
| PAS2-1R | CGTTTTAAAACCTAAGAGTCAC |
| PB42ADF | CCAGCCTCTTGCTGAGTGGAGATG |
| PB42ADR | AAGCCGACAACCTTGATTGGAG |
| PBABE3 | ACCCTAACTGACACACATTCC |
| pBABE5 | CTTTATCCAGCCCTCAC |
| PBAD-F | ATGCCATAGCATTTTTATCC |
| PBAD-R | ATCTGTATCAGGCTGAAAATC |
| PBRrevBam | GGTGATGTCGGCGATATAGG |
| PBV220+ | GGGCAGCATTCAAAGCAG |
| PBV220- | TTATCAGACCGCTTCTGC |
| PCDNA3.0F-F2 | CTCTGGCTAACTAGAGAACC |
| PCDNA3.0R-R2 | GGCAACTAGAAGGCACAGTC |
| PCDNA3.1F | CTAGAGAACCCACTGCTTAC |
| PCDNA3.1R | TAGAAGGCACAGTCGAGG |
| PCEP-F | AGAGCTCGTTTAGTGAACCG |
| pCMV5-F | TCTAAAAGCTGCGGAATTGT |
| pCMV5-R | TCCAAACTCATCAATGTATC |
| PDC316-F | ACGTGGGTATAAGAGGCG |
| PDC316-R | CGATGCTAGACGATCCAG |
| pDSRED-C1-F | AGCTGGACATCACCTCCCACAACG |
| pDsRed1-N | GTACTGGAACTGGGGGGACAG |
| pDonr-For | CTGGCAGTTCCCTACTCTCG |
| pDonr-Rev | TGTAACATCAGAGATTTTGAGACAC |
| PEF-F | TCAAGCCTCAGACAGTGGTTC |
| pEGFP-N5 | CGGTGGGAGGTCTATATAAG |
| pEGFP-N3 | GTCGCCGTCCAGCTCGACCAG |
| pEGFP-C5 | CATGGTCCTGCTGGAGTTCGT |
| pEGFP-C3 | TATGTTTCAGGTTCAGGG |
| PETBlueDOWN | ATAGCTTTAATGCGGTAGTT |
| pETUpstream | ATGCGTCCGGCGTAGA |
| pFastBac-F | TGAAGTGGTTCGCATCCTC |
| pFastBac-R | TGGACAAACCACAACTAGAATG |
| pFastBac-PH | AAATGATAACCATCTCGC |
| pGAPforward | GTCCCTATTTCAATCAATTGAA |
| pGEX5 | GCAAGCCACGTTTGGTG |
| PGEX3 | GAGCTGCATGTGTCAGAGG |
| pGL3+ | TAGCAAAATAGGCTGTCCC |
| pGL3- | CTTTATGTTTTTGGCGTCTTC |
| PinPoint-Primer | CGTGACGCGGTGCAGGGCG |
| PIRES2-EGFP.P5 | GTAGGCGTGTACGGTGGGAG |
| PIRES2-EGFP.P3 | AACGCACACCGGCCTTATTC |
| pFlag-CMV-F | GGTAGGCGTGTACGGTGG |
| pFlag-CMV-R | GCACTGGAGTGGCAACTT |
| pJET1.2F | CGACTCACTATAGGGAGAGCGGC |
| pJET1.2R | AAGAACATCGATTTTCCATGGCAG |
| pLEXA-F | CGTCAGCAGAGCTTCACCAT |
| pLEXA-R | TAAAACCTAAGAGTCACTTT |
| pLNCX-F | AGCTCGTTTAGTGAACCGTCAGATCG |
| pLNCX-R | ACCTACAGGTGGGGTCTTTCATTCCC |
| pLVX-R | ACTTGTGGCCATTCACAT |
| PLXSN-5 | CCCTTGAACCTCCTCGTTCGACC |
| PLXSN-3 | GAGCCTGGGGACTTTCCACACCC |
| PPC86-F | TATAACGCGTTTGGAATCACT |
| PPC86-R | GTAAATTTCTGGCAAGGTAGAC |
| pQE30+ | GTGAGCGGATAACAATTTCAC |
| pQE30- | CTGAACAAATCCAGATGGAG |
| pRNA-U6Forward | TACGATACAAGGCTGTTAGAGAG |
| psi-check2-F | ATGGGTAAGTACATCAAGAG |
| psi-check2-R | GAGGTCCGAAGACTCATTTA |
| PSilencer4.1-F | AGGCGATTAAGTTGGGTA |
| PSilencer4.1-R | CGGTAGGCGTGTACGGTG |
| pShuttle-CMV-F | GGTCTATATAAGCAGAGCTG |
| pShuttle-CMV-R | GTGGTATGGCTGATTATGATCAG |
| PT5 | TCCGAGATCTGGACGAGC |
| pTarget.F | CCAGGATTTTCCCAGTCAC |
| pTarget.R | GGCTTTACACTTTATGCTTC |
| PTARGET-SEQ | TTACGCCAAGTTATTTAGGTGACA |
| PTRC99C-F | TTGCGCCGACATCATAAC |
| PTRC99C-R | CTGCGTTCTGATTTAATCTG |
| PTRCHISA-F | CATGGTATGGCTAGCATGAC |
| PTRCHISA-R | CAGGCTGAAAATCTTCTCTC |
| RFP-Nrev | GTTCACGGTGCCCTCC |
| RV3 | CTAGCAAAATAGGCTGTCCC |
| RV4 | GACGATAGTCATGCCCCGCG |
| S1 | CAACGTGAAAAAATTATTATTCGC |
| S6 | GTAAATGAATTTTCTGTATGAGG |
| SP6 | ATTTAGGTGACACTATAGAA |
| S-TAG | CGAACGCCAGCACATGGACA |
| SV40 | GGAGGCTTTTTTGGAGGC |
| T7 | TAATACGACTCACTATAGG |
| T3 | ATTAACCCTCACTAAAGG |
| T7-TER | GCTAGTTATTGCTCAGCGG |
| TRX-FORWARD | TTCCTCGACGCTAACCTG |
| TRX-REVERSE | TGTAAAACGACGGCCAGTGC |
| TRX-F | CATATGAGCGATAAAATTATTCAC |
| TRX-R | GGATCCCTTGTCATCGTCATCACCA |
| U6-renyuan | ATGGACTATCATATGCTTACCGTA |
| U6-shuyuan | CAGTGCAGGGGAAAGAATAGTAGAC |
| U6-PROFOR | CCGTAACTTGAAAGTATTTCG |
| 2.0revprimer | AGGCGATTAAGTTGGGTA |
| 3.0rev | GAGTTAGCTCACTCATTAGGC |
| 5TriplEx2 | CTCCGAGATCTGGACGAGC |
| 17BASE | ACATCCACTTTGCCTTTCTC |
| Amp-F | TGAGATCCAGTTCGATGTAA |
| Amp-R | TCAGGCAACTATGGATGAAC |
| Kan-F | ATTCTCACCGGATTCAGT |
| Kan-R | GTCTGACCATCTCATCTGTA |
| pCold-F | GTAAAGCACGCCATATCGC |
| pCold-R | CCAAATGGCAGGGATCTTAG |
| PA | AGAGTTTGATCCTGGCTCAG |
| PB | AGGAGGTGATCCAGCCGC |
| PLL3.7-F | AACTATAGAGGCTTAATGTG |
| puc57-F | GCCATTCGCCATTCAGG |
| puc57-R | TAGCTCACTCATTAGGCAC |
| Kan-P1 | AACTCATCGAGCATCAAATG |
| Kan-P2 | CTCTAGGCCGCGATTAAATTC |
| 3C-forward | CTTCCTGTTAGTTAGTTACTTAAGC |
| 3creverse | GTAACATCAGAGATTTTGAGACAC |
| PCAG-F | GCAACGTGCTGGTTATTGTG |
| PTRCHISA-RV | ATCTGTATCAGGCTGAAAATC |
| PBAD-RV | ATCTGTATCAGGCTGAAAATC |
| PYES2-R | GCGTGAATGTAAGCGTGAC |
| ITS3 | GCATCGATGAAGAACGCAGC |
| V5reverse | ACCGAGGAGAGGGTTAGGGAT |
| L14724 | GACTTGAAAAACCACCGTTG |
| H15915 | CTCCGATCTCCGGATTACAAGAC |
| EF-1aF | TCAAGCCTCAGACAGTGGTTC |
| PLKO.1-5 | GACTATCATATGCTTACCGT |
| Lco1490 | GGTCAACAAATCATAAAGATATTGG |
| Hco2198 | TAAACTTCAGGGTGACCAAAAAATCA |
| WPRE-R | CATAGCGTAAAAGGAGCAACA |
| LR0R | GTACCCGCTGAACTTAAGC |
| LR5 | ATCCTGAGGGAAACTTC |
| PCAGGS-F | ATGTTCATGCCTTCTTCTTT |
| PCAGGS-R | TTCCTTTATTAGCCAGAAGT |
| YSFF | GAGCCAGTGAGTTGATTGTGT |
| YSRR | AGGCTTTTGACTTTCTGCTATGGAC |
| SV40pA-R | GAAATTTGTGATGCTATTGC |
| pENTR-F | CTACAAACTCTTCCTGTTAGTTAG |
| pENTR-R | ATGGCTCATAACACCCCTTG |
| IRES-R | CCTCACATTGCCAAAAGACG |
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