HBSS缓冲液用途和配方

Hanks’ 平衡盐溶液 (HBSS) 适用于各种细胞培养应用,例如解离前清洗细胞、运输细胞或组织、稀释细胞进行计数和制备试剂。配方含钙和镁,通常用作运输培养基或用于试剂制备。

 

组份 分子量 浓度Concentration (mg/L) mM
Inorganic Salts
Calcium Chloride (CaCl2) (anhyd.) 111.0 140.0 1.2612612
Magnesium Chloride (MgCl2-6H2O) 203.0 100.0 0.49261084
Magnesium Sulfate (MgSO4-7H2O) 246.0 100.0 0.40650406
Potassium Chloride (KCl) 75.0 400.0 5.3333335
Potassium Phosphate monobasic (KH2PO4) 136.0 60.0 0.44117647
Sodium Bicarbonate (NaHCO3) 84.0 350.0 4.1666665
Sodium Chloride (NaCl) 58.0 8000.0 137.93103
Sodium Phosphate dibasic (Na2HPO4) anhydrous 142.0 48.0 0.33802816
Other Components
D-Glucose (Dextrose) 180.0 1000.0 5.5555553
Phenol Red 376.4 10.0 0.026567481