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COVID19实时定量PCR检测序列和引物

名称描述引物序列(5’>3’)标记使用浓度
2019-nCoV_N1-F2019-nCoV_N1 Forward Primer5’-GAC CCC AAA ATC AGC GAA AT-3’None20 µM
2019-nCoV_N1-R2019-nCoV_N1 Reverse Primer5’-TCT GGT TAC TGC CAG TTG AAT CTG-3’None20 µM
2019-nCoV_N1-P2019-nCoV_N1 Probe5’-FAM-ACC CCG CAT TAC GTT TGG TGG ACC-BHQ1-3’FAM, BHQ-15 µM
2019-nCoV_N2-F2019-nCoV_N2 Forward Primer5’-TTA CAA ACA TTG GCC GCA AA-3’None20 µM
2019-nCoV_N2-R2019-nCoV_N2 Reverse Primer5’-GCG CGA CAT TCC GAA GAA-3’None20 µM
2019-nCoV_N2-P2019-nCoV_N2 Probe5’-FAM-ACA ATT TGC CCC CAG CGC TTC AG-BHQ1-3’FAM, BHQ-15 µM
2019-nCoV_N3-F2019-nCoV_N3 Forward Primer5’-GGG AGC CTT GAA TAC ACC AAA A-3’None20 µM
2019-nCoV_N3-R2019-nCoV_N3 Reverse Primer5’-TGT AGC ACG ATT GCA GCA TTG-3’None20 µM
2019-nCoV_N3-P2019-nCoV_N3 Probe5’-FAM-AYC ACA TTG GCA CCC GCA ATC CTG-BHQ1-3’FAM, BHQ-15 µM
RP-FRNAse P Forward Primer5’-AGA TTT GGA CCT GCG AGC G-3’None20 µM
RP-RRNAse P Reverse Primer5’-GAG CGG CTG TCT CCA CAA GT-3’None20 µM
RP-PRNAse P Probe5’-FAM – TTC TGA CCT GAA GGC TCT GCG CG – BHQ-1-3’FAM, BHQ-15 µM

1. ORF1 a/b序列
ATCGTGTTGTCTGTACTGCCGTTGCCACATAGATCATCCAAATCCTAAAGGATTTTGTGACTTAAAAGGTAAGTATGTACAAATACCTACAACTTGTGCTAATGACCCTGTGGGTTTTACACTTAAAAACACAGTCTGTACCGTCTGCGGTATGTGGAAAGGTTATGGCTGTAGTTGTGATCAACTCCGCGAACCCATGCTTCAGTCAGCTGATGCACAATCGTTTTTAAACGGGTTTGCGGTGTAAGTGCAGCCCGTCTTACACCGTGCGGCACAGGCACTAGTACTGATGTCGTATACAGGGCTTTTGACATCTACAATGATAAAGTAGCTGGTTTTGCTAAATTCCTAAAAACTAATTGTTGTCGCTTCCAAGAAAAGGACGAAGATGACAATTTAATTGATTCTTACTTTGTAGTTAAGAGACACACTTTCTCTAACTACCAACATGAAGAAACAATTTATAATTTACTTAAGGATTGTCCAGCTGTTGCTAAACAT
2. N基因
ATGTCTGATAATGGACCCCAAAATCAGCGAAATGCACCCCGCATTACGTTTGGTGGACCCTCAGATTCAACTGGCAGTAACCAGAATGGAGAACGCAGTGGGGCGCGATCAAAACAACGTCGGCCCCAAGGTTTACCCAATAATACTGCGTCTTGGTTCACCGCTCTCACTCAACATGGCAAGGAAGACCTTAAATTCCCTCGAGGACAAGGCGTTCCAATTAACACCAATAGCAGTCCAGATGACCAAATTGGCTACTACCGAAGAGCTACCAGACGAATTCGTGGTGGTGACGGTAAAATGAAAGATCTCAGTCCAAGATGGTATTTCTACTACCTAGGAACTGGGCCAGAAGCTGGACTTCCCTATGGTGCTAACAAAGACGGCATCATATGGGTTGCAACTGAGGGAGCCTTGAATACACCAAAAGATCACATTGGCACCCGCAATCCTGCTAACAATGCTGCAATCGTGCTACAACTTCCTCAAGGAACAACATTGCCAAAAGGCTTCTACGCAGAAGGGAGCAGAGGCGGCAGTCAAGCCTCTTCTCGTTCCTCATCACGTAGTCGCAACAGTTCAAGAAATTCAACTCCAGGCAGCAGTAGGGGAACTTCTCCTGCTAGAATGGCTGGCAATGGCGGTGATGCTGCTCTTGCTTTGCTGCTGCTTGACAGATTGAACCAGCTTGAGAGCAAAATGTCTGGTAAAGGCCAACAACAACAAGGCCAAACTGTCACTAAGAAATCTGCTGCTGAGGCTTCTAAGAAGCCTCGGCAAAAACGTACTGCCACTAAAGCATACAATGTAACACAAGCTTTCGGCAGACGTGGTCCAGAACAAACCCAAGGAAATTTTGGGGACCAGGAACTAATCAGACAAGGAACTGATTACAAACATTGGCCGCAAATTGCACAATTTGCCCCCAGCGCTTCAGCGTTCTTCGGAATGTCGCGCATTGGCATGGAAGTCACACCTTCGGGAACGTGGTTGACCTACACAGGTGCCATCAAATTGGATGACAAAGATCCAAATTTCAAAGATCAAGTCATTTTGCTGAATAAGCATATTGACGCATACAAAACATTCCCACCAACAGAGCCTAAAAAGGACAAAAAGAAGAAGGCTGATGAAACTCAAGCCTTACCGCAGAGACAGAAGAAACAGCAAACTGTGACTCTTCTTCCTGCTGCAGATTTGGATGATTTCTCCAAACAATTGCAACAATCCATGAGCAGTGCTGACTCAACTCAGGCCTAA
3. E 基因
ATGTACTCATTCGTTTCGGAAGAGACAGGTACGTTAATAGTTAATAGCGTACTTCTTTTTCTTGCTTTCGTGGTATTCTTGCTAGTTACACTAGCCATCCTTACTGCGCTTCGATTGTGTGCGTACTGCTGCAATATTGTTAACGTGAGTCTTGTAAAACCTTCTTTTTACGTTTACTCTCGTGTTAAAAATCTGAATTCTTCTAGAGTTCCTGATCTTCTGGTCTAA

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