常见的大肠杆菌细胞株特征和用途

 

细胞株名称 Natural resistance Primary Use 基因型/Genotype
ccdB Survival 2 T1R TOP10 derivative. For propagating plasmids expressing the ccdB gene (important in Gateway cloning). FmcrA Δ(mrr-hsdRMS-mcrBC) Φ80lacZΔM15 ΔlacX74 recA1 araΔ139 Δ(ara-leu)7697 galU galK rpsL (StrR) endA1 nupG fhuA::IS2
DB3.1 Streptomycin HB101 derivative. For propagating plasmids expressing the ccdB gene (important in Gateway cloning). F- gyrA462 endA1 glnV44 Δ(sr1-recA) mcrB mrr hsdS20(rB, mB) ara14 galK2 lacY1 proA2 rpsL20(Smr) xyl5 Δleu mtl1
DH10B Streptomycin MC1061 derivative. General cloning and storage, blue/white screening, leucine auxotroph. F endA1 recA1 galE15 galK16 nupG rpsL ΔlacX74 Φ80lacZΔM15 araD139 Δ(ara,leu)7697 mcrA Δ(mrr-hsdRMS-mcrBC) λ
DH5alpha General cloning and storage of common plasmids, blue/white screening. F endA1 glnV44 thi-1 recA1 relA1 gyrA96 deoR nupG Φ80dlacZΔM15 Δ(lacZYA-argF)U169, hsdR17(rK mK+), λ–
HB101 Streptomycin Hybrid of E. coli K12 and E. coli B (mostly K12, though), common lab strain for cloning and storage of pBR322 plasmids. F mcrB mrr hsdS20(rB mB) recA13 leuB6 ara-14 proA2 lacY1 galK2 xyl-5 mtl-1 rpsL20(SmR) glnV44 λ
JM109 General cloning and plasmid maintenance, blue/white screening, partly restriction-deficient; good strain for cloning repetitive DNA. endA1 glnV44 thi-1 relA1 gyrA96 recA1 mcrB+ Δ(lac-proAB) e14- [F’ traD36 proAB+ lacIq lacZΔM15] hsdR17(rKmK+)
JM110 Streptomycin For storing plasmids that should not be Dam or Dcm methylated, allows for methylation sensitive restriction enzymes to cut the plasmid after preparation. rpsL thr leu thi lacY galK galT ara tonA tsx dam dcm glnV44 Δ(lac-proAB) e14- [F’ traD36 proAB+ lacIq lacZΔM15] hsdR17(rKmK+)
MC1061 Streptomycin Parent of DH10B/TOP10 and derived strains, common lab cloning and storage strain. F Δ(ara-leu)7697 [araD139]B/r Δ(codB-lacI)3 galK16 galE15 λ e14 mcrA0 relA1 rpsL150(strR) spoT1 mcrB1 hsdR2(rm+)
MG1655 Wild type K-12 strain; first published sequence of an E. coli K-12 strain. F λ ilvG- rfb-50 rph-1
NEB Stable For cloning into and storage of lentiviral and retroviral vectors or cloning or repeated sequences with the potential to recombine. F’ proA+B+ lacIq ∆(lacZ)M15 zzf::Tn10 (TetR) ∆(ara-leu) 7697 araD139 fhuA ∆lacX74 galK16 galE15 e14-  Φ80dlacZ∆M15 recA1 relA1 endA1 nupG rpsL (StrR) rph spoT1 ∆(mrr-hsdRMS-mcrBC)
Pir1 For cloning and maintenance of a plasmids with R6Kγ origin; contains a mutant allele of the pir gene that maintains the plasmids at ~250 copies per cell. F- ∆lac169 rpoS(Am) robA1 creC510 hsdR514 endA recA1 uidA(∆MluI)::pir-116
Stbl2 JM109-derived. For cloning into and storage of lentiviral and retroviral vectors or cloning or repeated sequences with the potential to recombine. F- endA1 glnV44 thi-1 recA1 gyrA96 relA1 Δ(lac-proAB) mcrA Δ(mcrBC-hsdRMS-mrr) λ
Stbl3 Streptomycin Derived from HB101. For cloning into and storage of lentiviral and retroviral vectors or cloning or repeated sequences with the potential to recombine. This strain is endA+, so column-based DNA preps require an additional wash step. F- glnV44 recA13 mcrB mrr hsdS20(rB-, mB-) ara-14 galK2 lacY1 proA2 rpsL20 xyl-5 leu mtl-1
Stbl4 Electrocompetent cells for cloning and storage of unstable DNA and lentiviral/retroviral vectors. Used by Addgene for pooled library amplification. F’ mcrA Δ(mcrBC-hsdRMS-mrr) recA1 endA1 gyrA96 galthi-1 supE44 λrelA1 Δ(lac-proAB)/F’ proAB+lacIqZΔM15 Tn10 (TetR)
TOP10 Streptomycin MC1061 derivative. General cloning and storage, blue/white screening. F- mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74 nupG recA1 araD139 Δ(ara-leu)7697 galE15 galK16 rpsL(StrR) endA1 λ
XL1 Blue Tetracycline Blue/white screening and routine cloning, nalidixic acid resistant. endA1 gyrA96(nalR) thi-1 recA1 relA1 lac glnV44 F'[ ::Tn10 proAB+ lacIq Δ(lacZ)M15] hsdR17(rK mK+)
XL10 Gold Tetracycline and Chloramphenicol High competency cloning and propagation of large plasmids, ligated DNA, and libraries, nalidixic acid resistant. endA1 glnV44 recA1 thi-1 gyrA96 relA1 lac Hte Δ(mcrA)183 Δ(mcrCB-hsdSMR-mrr)173 tetR F'[proAB lacIqZΔM15 Tn10(TetR Amy CmR)]

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